人口样品的最佳储存时间和温度,以尽量减少微生物组的变化:一个范围审查
Hyun Ju Kim1,2, Dong Hyun Park3, Seung Hyun Han3
1Department of Periodontics, Seoul National University Dental Hospital, 101 Daehakro, Jongno-gu, Seoul 03080, Republic of Korea.
The Japanese dental science review
|November 5, 2024
概括
口腔微生物组样本的最佳储存包括在-80°C下立即冷,最长可达1-2年. 在适当的介质中,在室温下存储是可行的短时间 (1-2周).
科学领域:
- 微生物学 微生物学
- 这是生物银行.
- 基因组学就是基因组学.
背景情况:
- 生物标本的完整性对于准确的口腔微生物组分析至关重要.
- 不适当的储存条件可以显著改变牙斑块和唾液中的微生物概况.
- 对这些特定样本类型的最佳储存参数的研究有限.
研究的目的:
- 为了确定最佳的储存时间和温度,以保存人类牙斑和唾液的微生物特征.
- 综合现有关于生物标本存储的证据,用于口腔微生物组研究.
主要方法:
- 对研究的系统性文献审查,调查牙斑块和唾液的储存时间和温度.
- 纳入标准侧重于检查储存后微生物特征完整性的研究.
- 从最初识别的5433项研究中,分析了12项研究的数据.
主要成果:
- 建议在 -80°C或更低的温度下立即冷斑块和唾液,直到DNA提取,并保存长达1-2年.
- 一些研究表明,在-20°C或-80°C以特定溶液 (乙醇,珠子溶液) 进行更短的储存时间 (例如6个月).
- 在合适的介质中储存1-2周的室温可能不会显著影响微生物组合.
结论:
- 立即冷至-80°C是口腔微生物组样本长期存储的首选方法.
- 需要进一步的随机对照试验来建立最终的储存指南,特别是更长时间的储存.
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