血激活水的预处理大大提高了对Proteus mirabilis生物膜的菌体活性
Akash Shambharkar1, Thomas P Thompson1, Laura A McClenaghan1
1School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK.
Biofilm
|November 5, 2024
概括
血激活水 (PAW) 与菌体结合,可以有效地对抗Proteus mirabilis生物膜. 序列治疗,首先是PAW,显著增强了抗生物膜活性,提供了一种针对抗生素耐药性的新方法.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 传染性疾病 传染性疾病
背景情况:
- 抗微生物耐药性需要新的抗菌和抗菌膜药物.
- 血激活水 (PAW) 和菌体是有希望的替代品.
- 导管相关的尿路感染 (CAUTIs) 通常是由像Proteus mirabilis这样的耐药细菌引起的.
研究的目的:
- 调查PAW和细菌菌体对P.奇迹生物膜的联合疗效.
- 为了比较不同的PAW生产方法.
- 为了确定PAW和菌体的最佳顺序应用.
主要方法:
- 使用两个冷等离子体放电设置生成PAW.
- 针对P. mirabilis浮游生物和生物膜培养物进行了抗菌活性测试.
- 在PAW中评估了菌体稳定性.
- 综合治疗和连续应用被评估.
主要成果:
- 与单个治疗相比,PAW和菌体组合显示出增强的抗生物膜活性.
- 在生物膜嵌入细胞中减少了大约4 log.
- 用PAW连续治疗,然后用菌素是比反向顺序更有效的.
结论:
- PAW增强了菌体活动,显著降低了生物膜活力和生物量.
- 这种组合方法为管理抗生素耐药性CAUTIs提供了一个有希望的策略.
- 顺序应用对于最大限度地提高PAW-菌体组合的抗生物膜疗效至关重要.
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