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使用可诱导的光定时表达系统可视化Ubiquitin-like 3的细胞内动力学

Yuka Terada¹, Kumi Obara¹, Yusuke Yoshioka²

¹Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan.

|November 5, 2024

概括

视觉化了类似于乌比奎丁的3 (UBL3) 蛋白质动态,揭示了它从细胞质到多胞体 (MVB) 的运动. 这种蛋白质在MVP局部化之前与α-tubulin结合,影响小细胞外囊泡 (sEV) 载荷.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
细胞外囊泡细胞外囊泡

背景情况:

外体 (小细胞外囊泡,SEVs) 中介细胞间的通信.
乌比奎丁类3 (UBL3) 是一种影响sEV蛋白分类的新型后翻译性修饰.
UBL3局限于多胞体 (MVB) 和血膜,并在sEV中释放.

研究的目的:

研究UBL3.3的细胞内动力学和时空定位.
在MBB进入之前了解UBL3的运动和基板协会.

主要方法:

在Tet-on监管下为UBL3构建基于光计时器的可视化系统.
活细胞成像用于跟踪UBL3的细胞内运动.
超分辨率显微镜分析UBL3与α-tubulin等基质的关联.

主要成果:

在合成后,UBL3最初在细胞质中分布.
UBL3逐渐定位到血中,然后积聚在MVB中.
在细胞质中,UBL3与α-tubulin形成复合体,然后将其运送到MVBs.

结论:

一个新的时空可视化系统阐明了UBL3的细胞内动态.
在MVB局部化之前,UBL3在细胞质中与α-tubulin结合.
这为研究UBL3交互在MVB排序和sEV发布之前提供了基础.

关键词:

光计时器光计时器多流体体 (MVBs) 是一种多流体体.翻译后修改 (PTM) 是一种方法.类似于乌比奎丁的3 (UBL3)

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