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相关概念视频

The Extracellular Matrix01:29

The Extracellular Matrix

8.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
8.9K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.3K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.3K
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

328
Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
328
Drug Distribution: Tissue Binding01:21

Drug Distribution: Tissue Binding

2.6K
Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...
2.6K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.6K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.1K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.1K

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相关实验视频

Updated: Jun 8, 2025

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

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细胞外矩阵以组织特定的方式限制纳米粒子扩散和细胞吸收.

Devorah Cahn1,2, Alexa Stern1, Michael Buckenmeyer2

  • 1Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, United States.

ACS nano
|November 5, 2024
PubMed
概括

纳米粒子涂层对于克服疾病中的细胞外基质 (ECM) 障碍至关重要. 最佳的聚乙烯糖醇 (PEG) 涂层策略可增强纳米粒子扩散和细胞吸收,改善治疗效果.

关键词:
通过PEGylation进行化.没有细胞化的ECM.细胞外矩阵是细胞外矩阵.多个粒子跟踪追踪.纳米颗粒药物输送方法

更多相关视频

Manufacture and Drug Delivery Applications of Silk Nanoparticles
09:03

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

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Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
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Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

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相关实验视频

Last Updated: Jun 8, 2025

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.0K
Manufacture and Drug Delivery Applications of Silk Nanoparticles
09:03

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

15.7K
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
11:19

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

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科学领域:

  • 生物材料科学 生物材料科学
  • 纳米技术纳米技术
  • 药物运输 药物运输 药物运输

背景情况:

  • 癌症等疾病中的细胞外矩阵 (ECM) 重塑阻碍了纳米粒子药物输送.
  • 了解特定于组织的ECM障碍对于有效的纳米粒子设计至关重要.

研究的目的:

  • 研究组织特异性细胞外基质 (ECM) 特性如何影响纳米粒子扩散和细胞吸收.
  • 确定聚乙烯甘醇 (PEG) 链长度和分支对纳米粒子穿透ECM障碍物的影响.

主要方法:

  • 利用光视频显微镜评估各种ECM中的纳米粒子扩散.
  • 采用流细胞计量来量化纳米颗粒的细胞吸收.
  • 评估了从肝脏,肺部和小肠子粘膜中的纯化原和脱细胞化ECM中的纳米粒子行为.

主要成果:

  • 与脱细胞化组织ECM相比,纯化原蛋白对纳米粒子扩散构成了显著的障碍.
  • 密集的聚乙烯甘醇 (PEG) 涂层在ECM中显著增强了纳米粒子扩散 (高达2000倍) 和细胞吸收 (高达5倍).
  • 纳米粒子的移动性和最佳的PEGylation策略因ECM类型和度而异.

结论:

  • 低分子量PEG涂层在ECM透和细胞吸收之间提供了平衡.
  • 分支PEG涂层在ECM透和细胞吸收方面表现出特定组织的增强.
  • 结果为设计纳米粒子提供了洞察力,这些纳米粒子能够有效地穿越特定组织的ECM障碍,从而提高治疗效率.