全球同调节交叉交谈m6A和m5C之间的RNA甲基化系统协调细胞反应和大脑疾病途径
Oliver Chukwuma Orji1,2, Joseph Stones1, Seema Rajani3
1Division of Cells, Organisms and Molecular Genetics, School of Life Sciences, University of Nottingham, Nottingham, NG7 2UH, UK.
Molecular neurobiology
|November 5, 2024
概括
N6腺 (m6A) 和C5细胞 (m5C) 的RNA甲基化系统相互作用,揭示了跨调节的反循环. 这些相互作用影响细胞过程,蛋白质组反应和大脑疾病机制.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- N6腺 (m6A) 和C5细胞 (m5C) 修饰是由不同的效应蛋白调节的.
- 这些效应蛋白通常被认为是独立运作的.
研究的目的:
- 研究m6A和m5CRNA甲基化系统之间的潜在交叉调控相互作用.
- 确定这些系统之间新的共同监管关系和功能连接.
主要方法:
- 对效应蛋白转录的相互基基修饰的分析.
- 全球质谱学生物干扰后的蛋白质组学.
- 同调节蛋白质的基因本体学分析.
- 在体外同位体定位测试.
主要成果:
- 全球跨调节相互作用和m6A和m5CRNA甲基化系统之间的功能联系的证据.
- 识别涉及效应蛋白转录的相互转录后反循环.
- 发现了新的共同调节性细胞反应,包括ALKBH5 (m6A擦拭器) 和NSUN4 (m5C写字器) 之间的反应.
- 在各种细胞过程中确定了跨系统的控制,如蛋白质体和线粒体机制,SUMOylation和酸化.
- 发现了新的效应蛋白网络关系,包括与智力障碍途径的联系.
- 在体外证实了m6A-RNAs和m5C阅读器蛋白ALYREF之间的同位化.
结论:
- m6A和m5CRNA甲基化系统并不独立,但表现出显著的交叉调节.
- 这些相互作用影响着各种细胞过程,蛋白质组反应,并与大脑疾病机制有关.
- 这些发现为了解RNA代谢及其在细胞功能和疾病中的作用提供了新的框架.
相关概念视频
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
Regulation of Expression at Multiple Steps
873
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
873
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Regulation of Expression Occurs at Multiple Steps
22.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.5K
Global Regulatory Systems
2
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
2
Regulated mRNA Transport
6.2K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.2K


