相关实验视频

Updated: Jun 8, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

在大米中使用SpCas12f进行农细菌介导的转化和向突变

Satoru Sukegawa¹, Seiichi Toki^1,2,3,4, Hiroaki Saika⁵

¹Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.

Methods in molecular biology (Clifton, N.J.)

|November 5, 2024

概括

我们介绍了使用紧的CRISPR-Cas12f系统在大米中进行向突变发生的协议. 这种微型的CRISPR-Cas12f基因编辑工具为植物的载体构造和转化效率提供了优势.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
植物科学植物科学

背景情况:

集群定期间隔的短平行体重复 (CRISPR) -Cas是一种转基因生物适应性免疫系统,用于基因组编辑.
克里斯普尔-Cas9被广泛使用,但它的尺寸可能会限制某些应用.
CRISPR-Cas12f是一种较小的替代方案,为矢量构造和转换效率提供了潜在的好处.

研究的目的:

描述使用Syntrophomonas palmitatica Cas12f (SpCas12f) 在大米中的向突变发生的协议.
突出使用紧的CRISPR-Cas12f系统用于植物基因组编辑的优点.
要总结增强SpCas12f介导向突变发生率的方法.

主要方法:

米的农细菌介导的转化.
使用Syntrophomonas palmitatica Cas12f (SpCas12f) 的有针对性的突变发生.
优化策略,以提高突变发生效率.

主要成果:

成功实施了一项用于大米中SpCas12f介导的向突变发生的协议.
展示了紧的CRISPR-Cas12f对植物基因组工程的有用性.
确定增加向突变频率的方法.

结论:

紧的SpCas12f系统是一种可行的和有利的工具,用于在米中进行基因组编辑.
该协议促进了植物有效的向突变发生.
进一步的优化策略可以增强CRISPR-Cas12f在植物生物技术中的应用.

关键词:

通过农业细菌介导的转化过程.这就是CRISPR-Cas12f.基因组编辑基因组编辑在SpCas12f中使用.有针对性的突变发生.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...