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相关概念视频

CRISPR01:59

CRISPR

49.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.8K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.8K
Homologous Recombination02:31

Homologous Recombination

50.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.2K
RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K

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相关实验视频

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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在大米中使用CRISPR/Cas-Mediated Base Editor进行精确的基础替换.

Masaki Endo1, Katsuya Negishi2, Seiichi Toki3,4,5,6

  • 1Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. mendo@affrc.go.jp.

Methods in molecular biology (Clifton, N.J.)
|November 5, 2024
PubMed
概括

这项研究介绍了SpCas9-NG,这是一款工程基因编辑器,可以扩展基因组编辑功能. 它使用轻松的NG原空间体相邻动图 (PAM) 来实现在更容易准的位置进行精确的基基转换,而不会造成DNA断裂.

关键词:
亚丁酸基编辑器的编辑器通过农业细菌介导的转化过程.替代基的替代基是什么这就是CRISPR/CasPR.细胞因子基编辑器

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科学领域:

  • 分子生物学分子生物学
  • 基因组工程是基因组工程.
  • 生物技术是生物技术.

背景情况:

  • 克里斯普尔/卡斯系统提供精确的基因组编辑,没有双链断裂.
  • 原始空间器相邻动图 (PAM) 序列限制了基础编辑器的可准范围.
  • 标准SpCas9需要一个NGG PAM,限制编辑网站.

研究的目的:

  • 描述使用工程 SpCas9-NG 变体编辑基础的协议.
  • 扩大基础编辑应用程序的灵活性和目标范围.
  • 为了在放松的NG PAM序列的位置上实现基替代.

主要方法:

  • 使用工程 SpCas9-NG 变体进行基础编辑.
  • 在目标DNA位点实施精确基因转换协议.
  • 利用更改的PAM识别来提高编辑可访问性.

主要成果:

  • SpCas9-NG 能够识别放松的 NG PAM 序列.
  • 扩大了基础编辑应用程序的目标范围.
  • 促进了精确的基因转换,而不会诱导DNA双链断裂.

结论:

  • 该SpCas9-NG基基编辑器增强了基于CRISPR的基因组编辑的多功能性.
  • 该协议允许通过克服PAM限制,更广泛地应用基础编辑.
  • 精确的基因组编辑通过放松的PAM识别变得更容易获得.