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Updated: Jun 8, 2025

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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DeLTa-Seq:大米叶的高通量向RNA-Seq,没有RNA净化

Makoto Kashima1, Yasuyuki Nomura2, Atsushi J Nagano3,4

  • 1Faculty of Science, Department of Molecular Biology, Toho University, Chiba, Japan.

Methods in molecular biology (Clifton, N.J.)
|November 5, 2024
PubMed
概括

直接lysate测序 (DeLTa-seq) 是一种新的RNA-seq方法,用于在不需要RNA净化的情况下精确量化基因表达. 这种高通量技术使用直接逆转录在叶溶解酸上进行高效的分析.

关键词:
直接-lysate反转录的反转录基因表达分析 基因表达分析高吞吐量RNA-seqq 的高吞吐量RNA-seq.叶子 叶子 叶子大米 大米 大米 大米 大米有针对性的RNA-seq.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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