Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecular basis underlying the isoprene emission diversity in Fagaceae.

Plant physiology·2026
Same author

Ethanol application enhances freezing stress tolerance in Arabidopsis and sugar beet.

Plant molecular biology·2026
Same author

Hybridization Drives High Genetic Diversity in the Japanese Fire-Bellied Newt, Cynops pyrrhogaster.

The Journal of heredity·2026
Same author

Revised taxonomy reveals sustained introgression and secondary contact in ancient lake ricefishes.

BMC ecology and evolution·2026
Same author

Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery.

Plant physiology·2026
Same author

Rice EMF3 Alleles Adjust Flower Opening Time to Enhance the Seed Setting Rate Under High Temperature Stress.

Plant biotechnology journal·2026

相关实验视频

Updated: Jun 8, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K

拉西-Seq:一个高通量3'RNA-Seq方法,用于大规模的转录基因组概况在米中.

Natsumi Mori-Moriyama1, Atsushi J Nagano2,3

  • 1Faculty of Agriculture, Ryukoku University, Shiga, Japan.

Methods in molecular biology (Clifton, N.J.)
|November 5, 2024
PubMed
概括

Lasy-Seq提供了一种低成本,简单的方法来准备3'RNA测序库. 这种高通量技术简化了样本索引和聚合,减少了数百个样本的处理时间.

关键词:
3′RNA-seqq3′RNA-seq3′RNA-seq3′RNA-seq3′RNA-seq3′RNA-seq3′ RNA-seq3′ 3′RNA-seq3′ 3′RNA-seq3′ 3′RNA-seq3′ 3′RNA-seq3′ 3′RNA-seq3′ 3′RNA-seq3′ 3′高吞吐量RNA-seqq 的高吞吐量RNA-seq.大规模的RNA-seqq.可扩展的RNA-seqq.转录组 转录组就是一个转录组.

更多相关视频

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

8.0K
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.8K

相关实验视频

Last Updated: Jun 8, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

8.0K
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.8K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • RNA测序 (RNA-seq) 对于基因表达分析至关重要.
  • 传统的图书馆准备方法可能耗时且昂贵.
  • 3'RNA-seq专注于转录的3'末端,提供了一个具有成本效益的方法.

研究的目的:

  • 介绍Lasy-Seq,一种新的,精简的库准备方法,用于3'RNA-seq.
  • 为了证明Lasy-Seq在高吞吐量应用中的效率和可扩展性.
  • 为了减少与RNA-seq库准备相关的成本和实践时间.

主要方法:

  • 拉西-Seq使用具有独特索引序列的oligo(dT) 开头.
  • 同时逆转录 (RT) 和样本索引是在单个步骤中执行的.
  • 在聚合样本中,后RT步骤被整合,从而消除了个别处理.

主要成果:

  • 通过Lasy-Seq,可以为3'RNA-seq.准备高吞吐量库.
  • 该方法允许同时进行RT和样本索引.
  • 复杂的图书馆池从数百个样本可以在几天内准备好.

结论:

  • Lasy-Seq显著简化和加速RNA-seq库准备工作流.
  • 这种方法适用于高效处理大量样品.
  • 拉西-Seq为3'RNA-seq.提供了一个具有成本效益和易于使用的替代方案.