膜和骨髓衍生的巨细胞在新陈代谢和谷氨酸利用上有所不同
Christine L Vigeland1,2, Jordan D Link1,2, Henry S Beggs1,2
1Division of Pulmonary Diseases and Critical Care Medicine, Department of Medicine.
American journal of respiratory cell and molecular biology
|November 5, 2024
概括
与骨髓衍生的巨细胞 (BMDM) 相比,组织居住的膜巨细胞 (AM) 呈现出不同的代谢概况. 谷氨胺代谢显著影响AM代谢反应,与BMDM不同.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞的新陈代谢
- 巨细胞生物学 巨细胞生物学
背景情况:
- 巨细胞的代谢活动决定了它们的表型和功能.
- 预炎性巨细胞利用糖解,而前修复性巨细胞则依赖氧化代谢和谷氨酸溶解.
- 以前的研究主要使用巨细胞系和骨髓衍生巨细胞 (BMDM).
研究的目的:
- 为了研究LPS刺激后在小鼠组织居住的膜巨细胞 (AM) 中的代谢活动变化.
- 为了比较AM与BMDM的代谢概况.
- 确定谷氨胺代谢在AM和BMDM中的作用.
主要方法:
- 对小鼠膜巨细胞 (AM) 和骨髓衍生巨细胞 (BMDM) 的代谢分析.
- 用脂聚糖 (LPS) 进行刺激.
- 使用6-diazo-5-oxo-L-norleucine (DON) 抑制谷氨胺代谢.
主要成果:
- 与BMDM相比,AM具有更高的氧气消耗率和更高的脂肪酸氧化代谢物度.
- 在BMDM中,LPS刺激增加了糖解,但在AM中没有.
- 通过DON抑制谷氨胺影响了AM代谢,增加了糖解,TCA循环,脂肪酸氧化和氨基酸代谢物,并在AM中改变了LPS反应,但不是BMDM.
结论:
- 鼠AM和BMDM在它们的代谢活动中表现出显著的差异.
- 谷氨胺代谢在调节AM代谢灵活性和对炎症刺激的反应方面发挥着至关重要的作用.
- 这些发现凸显了组织居民和骨髓衍生的巨细胞之间独特的代谢调节策略.
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