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使用RNA测序对具有不同DNA碎片化指数的人类精子进行转录组分析

Kailin Yang¹, Xue Sun², Qiyuan Zheng¹

¹Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.

Reproductive biology

|November 5, 2024

概括

男性的精子DNA碎片化与活性氧物种 (ROS) 的增加和线粒体潜力的降低有关. 基因表达分析显示,与氧化应激和精子生成相关的关键基因在高DNA碎片化的精子中被下调.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
氧化压力研究研究氧化压力研究

背景情况:

精子DNA碎片化 (SDF) 对男性生育能力和辅助生殖结果产生负面影响.
了解SDF背后的分子机制对于改善精子选择和治疗策略至关重要.

研究的目的:

在男性中识别与精子DNA碎片化 (SDF) 相关的差异表达基因 (DEG).
提供基于DNA碎片化水平的辅助生殖中精子选择的分子基础.

主要方法:

评估了60个精液样本,按高,中,低精子DNA碎片化指数 (DFI) 分类.
使用多色流细胞计,评估活性氧物种 (ROS) 含量,酸 (PS) 外化和线粒体膜潜力 (MMP).
RNA测序 (RNA-seq),其次是基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG). DEGs的途径分析.
使用定量逆转录PCR (qRT-PCR) 验证关键的DEG.

主要成果:

精子具有更高的DFI表现出增加的ROS含量和PS外部化,以及减少的MMP.
通过RNA-seq,在高,中,低DFI群体中确定了5334个DEG.
GO和KEGG分析显示,高DFI组中的421个下调基因与氧化应激和精子生成有关.
通过qRT-PCR识别和验证了13个涉及SDF的新型基因.

结论:

高度碎片化的精子DNA与对抗氧化剂防御和精子生成至关重要的基因的下调有关.
已确定的DEG为评估精子质量和指导辅助生殖技术提供了潜在的生物标志物.

关键词:

氧化应激是一种氧化应激.在RNA-seqqq.精子DNA碎片化的情况精子质量精子质量

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