在小麦中使用拉曼光谱学结合化学测量来确定阿弗拉托克辛B1
Congli Mei1, Ziyu Wang2, Hui Jiang2
1College of Electrical Engineering, Zhejiang University of Water Resources and Electric Power, Hangzhou 310048, PR China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|November 5, 2024
概括
这项研究提出了一种快速检测小麦中的致癌性阿弗拉托辛B1 (AFB1) 的方法,使用拉曼光谱和化学测量. 开发的模型准确量化了AFB1污染,确保了食品安全.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 频谱学是一种光谱学.
背景情况:
- 甲素B1 (AFB1) 是一种强有力的致癌物质,经常作为小麦中的污染物被发现.
- 准确和快速检测小麦中AFB1的方法对于食品安全和质量控制至关重要.
研究的目的:
- 开发和验证使用拉曼光谱法在小麦中检测AFB1的定量检测方法.
- 评估化学测量技术在提高AFB1量化的准确性方面的有效性.
主要方法:
- 小麦样本的原始拉曼光谱使用Savitzky-Golay平滑和基线校正进行了预处理.
- 使用竞争性自适应重量化抽样 (CARS),代变量子集优化 (IVSO) 和引导软收缩 (BOSS) 进行了变量选择.
- 构建了部分最小平方回归 (PLSR) 模型来量化AFB1水平.
主要成果:
- 所有测试的变量优化方法都改善了模型预测性能.
- 启动软收缩-部分最小平方回归 (BOSS-PLSR) 模型表现出了出色的概括性和稳定性.
- BOSS-PLSR模型实现了0.9927的预测确定系数 (RP2),2.4260μg/kg的RMSEP和11.5250.0的RPD.
结论:
- 拉曼光谱和化学测量的结合方法为在小麦中快速定量检测AFB1提供了可靠的方法.
- 这种技术为食品中AFB1污染的常规监测提供了一个有希望的解决方案.
相关概念视频
01:20Raman Spectroscopy: Overview
313
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
313
01:26Raman Spectroscopy Instrumentation: Overview
296
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
296