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体编码的酸酶RapP增强了非化Bacillus subtilis菌株的细胞生长

Manlu Zhu¹, Yiheng Wang¹, Haoyan Mu¹

¹State Key Laboratory of Green Pesticide; Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, PR China.

Nature communications

|November 5, 2024

概括

细菌细菌的等离子体pBS32,通过RapP酸酶,通过延迟适应性反应来增强生长. 这样可以优化资源配置,以改善健康状况,而不会牺牲长期生存.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌生理学细菌生理学

背景情况:

微生物健康受到快速生长和其他基本特征 (如生存和适应能力) 之间的权衡的限制.
从马尔堡菌株化出来的Bacillus subtilis实验室菌株已经丢失了编码RapP酸酶的等离子体pBS32.
拉普P去化Spo0F,调节关键的发育途径,如生物膜形成和分泌.

研究的目的:

研究细菌细菌等离子体pBS32及其编码的RapP酸酶在调节生长和健康方面的作用.
了解RapP在指数增长过程中如何影响Spo0F-Spo0A信号通路.
确定RapP是否有助于管理生理性权衡,以提高细胞资源分配.

主要方法:

用和没有等离子体的B. subtilis菌株的比较分析pBS32.
分子测定用于监测Spo0F酸化状态和Spo0A激活.
蛋白质组分析,以评估资源在增长和适应途径之间的重新分配.
在指数和静止阶段的增长率测量.

主要成果:

等离子体pBS32,特别是rapP基因,显著提高了细菌的生长率.
在指数增长阶段,RapP可以防止Spo0F-Spo0A适应反应过早激活.
这种延迟允许细胞资源更多地重新分配到生物合成和促进生长的途径.
在静止阶段没有观察到长期健康状况的妥协.

结论:

由等离子体pBS32编码的RapP酸酶对于优化Bacillus subtilis生长至关重要.
通过管理快速增长和适应性反应之间的权衡,RapP促进了健康的最大化.
通过延迟应激反应节约细胞资源,可以改善生长,而不会影响静止阶段的生存.

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