相关实验视频

Updated: Jun 8, 2025

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

单细胞RNA测序分析免疫和介质细胞交叉在发育中的化

Valia P Leifer¹, Fei Fang², Lee Song¹

¹Department of Orthopedic Surgery, Columbia University, New York, NY, 10032, USA.

Scientific reports

|November 5, 2024

概括

免疫细胞 (ICs) 在化发育过程中与介质细胞 (MCs) 相互作用. 这些相互作用对化矿化至关重要,可能会限制自身免疫力,为化病提供新的见解.

科学领域:

免疫学免疫学免疫学
发展生物学发展生物学
类风湿病学类风湿病学

背景情况:

诸如肠道病症之类的自身免疫性疾病涉及免疫细胞透和在肠道处的失调.
虽然免疫细胞存在于健康的体中,但它们在发育过程中的作用仍然是未知的.

研究的目的:

在化发育过程中对免疫细胞 (IC) 亚型进行表征.
研究ICs和介质细胞 (MCs) 之间的相互作用.
了解这些相互作用在化矿化和自身免疫中的作用.

主要方法:

单细胞RNA测序分析不同矿化阶段的内组织.
在ICs和MCs之间监管相互作用的推断.

主要成果:

鉴定了九种不同的IC亚型 (B细胞,巨细胞,中性粒细胞,T细胞) 在中.
证据表明ICs促进了MC的扩散和差异化.
证明调节IC表型和自身免疫的MCs.
建议ICs和MCs之间的双向监管相互作用影响矿物化.

结论:

集成电池和集成电池之间的双向通信对于矿化至关重要.
原生MCs具有在发育过程中抑制自身免疫的独特能力.
这些发现提供了对体发育和自身免疫性疾病病原体的新见解.

关键词:

细胞细胞交叉的交叉.总结论文的发展.免疫细胞是免疫细胞.介质细胞是介质细胞.矿物化的矿物化.单细胞RNA测序的测序方法

更多相关视频

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...