基于ATAC-seq和RNA-seq的综合分析揭示了肝细胞癌中一种新型的瘤基因PRPF3
Yi Bai1, Xiyue Deng2, Dapeng Chen2
1Department of Hepatobiliary Surgery, School of Medicine, Tianjin First Central Hospital, Nankai University, Tianjin, China.
Clinical epigenetics
|November 5, 2024
概括
这项研究揭示了ZNF93通过调节新型瘤基因PRPF3.3,促进肝细胞癌 (HCC) 的进展. 研究结果强调ZNF93和PRPF3是HCC治疗的关键分子标.
科学领域:
- 基因组学和表观遗传学
- 癌症生物学 癌症生物学
- 分子瘤学分子瘤学
背景情况:
- 转移酶可访问染色体测序 (ATAC-seq) 的测试确定了对基因调节至关重要的开放染色体区域.
- 肝细胞癌 (HCC) 的发展受到染色质可访问性和基因表达的改变的影响.
- 了解这些表观遗传变化对于确定HCC治疗点至关重要.
研究的目的:
- 通过使用ATAC-seq.识别调节HCC发育和进展的关键分子标.
- 阐明驱动HCC的潜在表观遗传机制.
- 调查在HCC.中发现的调节因素的功能作用.
主要方法:
- 使用ATAC-seq.比较HCC和正常组织之间的染色质可访问性概况.
- 整合ATAC-seq与RNA-sequencing (RNA-seq) 来识别差异表达基因 (DEG) 和差异可访问区域 (DAR).
- 进行了功能实验,以评估已识别的因素对HCC细胞增殖和迁移的影响.
主要成果:
- 独特的信号通路和转录因子与HCC中的DAR相关.
- 上调的DAR与MAPK和FoxO通路相关;下调的DAR与TGF-β,cAMP和p53通路相关.
- 鉴定出PRPF3是一种促进HCC增殖和迁移的新型瘤基因,受到ZNF93.3.的调控.
结论:
- 通过对瘤基因PRPF3.3的转录调节,ZNF93促进HCC细胞的增殖和运动.
- 这项研究提供了对HCC.的综合表观遗传景观分析.
- 突出ZNF93和PRPF3作为肝细胞癌的潜在治疗点.
相关概念视频
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...