野猪丸中的生物因子和风险因素以及与精子细胞运动性的关系
Marko Halo1, Filip Tirpák2, Michal Miškeje3
1Institute of Applied Biology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
概括
环境污染影响了野猪的繁殖. 这项研究发现和等有毒元素在丸中与精子运动率降低相关,突出显示它们作为生物指标的作用.
科学领域:
- 环境毒理学环境毒理学
- 野生动物生殖健康野生动物生殖健康
- 生物监测 生物监测
背景情况:
- 环境污染对野生动物造成重大健康风险,通过组织中元素的积累影响生殖能力.
- 评估动物组织中的元素度可以了解环境污染及其生物影响.
研究的目的:
- 为了研究野猪丸中选定的元素的度.
- 为了评估与元素度相关的 epididymal 精子细胞的运动性.
- 为了确定特定元素和野猪的繁殖参数之间的潜在相关性.
主要方法:
- 从斯洛伐克共和国的Žuhračka - Levice地区采集了野猪样本.
- 测试是在死后收集的,精子从尾巴上分离出来,用于计算机辅助精子分析 (CASA).
- 在丸中的元素度使用诱导合等离子体 (ICP) 和冷蒸汽原子吸收光谱法 (CV-AAS) 来量化.
主要成果:
- 精子细胞的运动率平均为44.29%,渐进性运动率为18.47%.
- 测试中的主要元素是K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > Cd > Li > Hg. 在测试中,主要元素是K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > Cd > Li > Hg.
- 在 (Se) 和总/渐进性运动性, (As) 和精子速度/节拍交叉频率,以及 (Hg) 和节拍交叉频率之间观察到显著的相关性.
结论:
- 野猪丸中积的有毒元素,如和,与精子运动能力受损有关.
- 野猪组织中的元素度可以作为环境污染及其对野生动物繁殖有害影响的敏感指标.
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