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菌根菌在呼吸道上皮质细胞上形成生物膜,并促进粘膜屏障的破坏
Amy M Barclay1, Dennis K Ninaber2, Ronald W A L Limpens3
1Leiden University Center for Infectious Diseases, (LUCID), Leiden University Medical Center, Leiden, the Netherlands.
iScience
|November 6, 2024
概括
结核菌可以在呼吸道细胞上形成生物膜,导致感染复发和抗生素耐药性. 这项研究揭示了这些生物膜如何影响表皮屏障功能并促进细胞死亡.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 传染性疾病 传染性疾病
背景情况:
- 结核病 (TB) 表现出生物膜相关感染的特征,如治疗耐药性和复发.
- 呼吸道上皮是对空气传播的病原体的关键防御,例如Mycobacterium tuberculosis (Mtb).
研究的目的:
- 研究不同菌根菌种在人类呼吸道上皮质细胞上形成生物膜的能力.
- 分析这些生物膜对表皮屏障完整性和细胞活性的影响.
主要方法:
- 使用一种空气-液体接口模型与初级支气管上皮细胞 (PBECs).
- 与四种真菌菌菌种共同培养的PBECs:Mtb,M. bovis (BCG),M. avium和M. smegmatis. 这四种真菌菌种.
- 评估生物膜形成,矩阵组成,代谢活动,上皮屏障破坏和细胞死亡.
主要成果:
- Mtb,BCG和M. smegmatis在PBEC上形成了具有细胞外基质的生物膜.
- 生物膜特性 (生物质,多糖含量,代谢活性) 在物种之间有所不同.
- 菌根菌暴露破坏了上皮屏障,并诱导了主要的非光性细胞死亡.
- 结构分析在7天的生物膜中发现了类似毛孔的结构.
结论:
- 菌根菌具有在人类呼吸道上皮质细胞上形成生物膜的能力.
- 长期的真菌细菌生物膜感染会损害上皮质屏障的功能.
- 菌根细菌的生物膜形成促进了上皮细胞的死亡,有助于疾病的发病.
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