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相关概念视频

What is Gene Expression?01:42

What is Gene Expression?

131.7K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
131.7K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.3K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.3K
Master Transcription Regulators02:23

Master Transcription Regulators

6.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.0K
Reporter Genes02:11

Reporter Genes

11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
What is Gene Expression?01:36

What is Gene Expression?

9.9K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
9.9K
Global Regulatory Systems01:28

Global Regulatory Systems

945
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
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相关实验视频

Updated: Apr 25, 2026

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

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基因监管网络的方法 基因监管网络的方法 基因监管的方法 基因监管的方法

Karamveer1, Yasin Uzun1,2,3

  • 1Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, USA.

Bioinformatics and biology insights
|November 6, 2024
PubMed
概括

这项研究解决了使用单细胞测序数据的基因调控网络 (GRN) 构建方法缺乏全面的基因基因基因测试. 它为评估这些计算方法提供了一个框架,这对于理解细胞分化至关重要.

关键词:
基因监管网络是基因监管网络.基准测试 (benchmarking) 是一种比较的方法.在表观基因组学上,表观基因组学.基本的真理 基本的真理单细胞基因组学 单细胞基因组学

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科学领域:

  • 计算生物学 计算生物学
  • 基因组学就是基因组学.
  • 系统生物学 系统生物学

背景情况:

  • 基因调控网络 (GRNs) 模拟控制基因表达和细胞分化的遗传相互作用.
  • 单细胞测序为构建GRN提供高分辨率数据.
  • 现有的GRN构建的计算方法缺乏标准化的基准测试.

研究的目的:

  • 为从单细胞数据中构建基因调控网络提供对基因调控网络构建的基准分析方法的全面讨论.
  • 建立标准化术语,黄金标准数据集和性能指标,用于评估GRN推理方法.

主要方法:

  • 对 GRN 构建和基准测试的现有文献进行审查和综合.
  • 定义GRN术语和共同的黄金标准数据集.
  • 识别和分析网络建设方法的性能指标.

主要成果:

  • 澄清GRN术语和共同的基准测试数据集.
  • 定义用于评估GRN推理算法的性能指标.
  • 讨论各种基准分析策略的优点和局限性.

结论:

  • 标准化对比方法对于从单细胞数据推进GRN构建至关重要.
  • 该研究为未来的方法开发和比较分析提供了基础.
  • 强调需要替代地面真相数据集,并进一步考虑对GRN推断的基准测试.