软组织肉瘤的外周免疫分析:疾病监测的前景
Jani Sofia Almeida1,2,3,4,5,6, Luana Madalena Sousa1,5,6, Patrícia Couceiro1,3,4,5,6
1Center for Neurosciences and Cell Biology (CNC), Laboratory of Immunology and Oncology, University of Coimbra, Coimbra, Portugal.
Frontiers in immunology
|November 6, 2024
概括
软组织肉瘤 (STS) 患者表现出受损的全身免疫力,免疫细胞简介发生变化,细胞毒性潜力降低. 周围免疫型与生存相关,为STS的结果预测提供了一种新方法.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 医学研究 医学研究
背景情况:
- 目前对软组织肉瘤 (STS) 的研究重点是瘤微环境和淋巴结.
- 对瘤样本的有限访问需要使用替代方法来评估非手术性STS患者的免疫反应.
研究的目的:
- 评估STS患者的周围免疫特征.
- 根据他们的免疫特征来表征STS患者.
- 探索外围免疫类型对患者存活率的影响.
主要方法:
- 收集了55名STS患者和年龄相匹配的健康捐赠者的血液样本.
- 使用多参数流细胞计和实时RT-qPCR对全血进行了深度免疫类型和基因表达分析.
- 使用xMAP技术对血样本进行蛋白质组分析.
- 使用无监督集群来分类患者并分析生存相关性.
主要成果:
- 与健康捐赠者相比,STS患者表现出显著的差异,包括减少B细胞和CD4T细胞,减少ICOSLG和CD40LG的表达.
- 观察到较高的抑制因子,如增加的M-MDSC和记忆Tregs,更高的ARG1表达,以及增加的血IL-10,可溶性VISTA和可溶性TIMD-4.
- 在NK和CD8T细胞中明显出现了细胞毒性潜力的损害,CD56dimNK细胞减少,PRF1,GZMB和KLRK1水平降低.
- 患者被分为"免疫性高"",免疫性中等"和"免疫性低"组,与生存结果相关.
结论:
- STS患者出现了系统免疫受损的情况.
- 外周免疫型与生存率之间的相关性凸显了在STS中分析外周血液的重要性.
- 评估周围免疫反应可能成为监测和预测STS患者结果的宝贵工具.
相关概念视频
02:07Tumor Progression
6.2K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.2K
01:27Tumor Immunotherapy
2.5K
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.5K