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功能性蛋白质与微纤维表面的共价粘附,通过对位点选择性四素结合的一般策略

Paramesh K Ramaraj¹, Mugdha Pol², Samuel L Scinto¹

¹Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States.

ACS applied materials & interfaces

|November 6, 2024

概括

一种新的Affinity Bioorthogonal Chemistry (ABC) 标签方法使蛋白质能够精确地附着在材料上. 这种技术保持了蛋白质的功能,并允许控制多蛋白质表面的修饰,促进生物材料的开发.

科学领域:

生物材料科学生物材料科学
化学生物学化学生物学
表面化学表面化学

背景情况:

用蛋白质修改表面对于各种生物应用至关重要.
现有的方法经常与不同结构,大小和功能的蛋白质作斗争.
存在对多功能和选择性蛋白质结合技术的需求.

研究的目的:

提出一种新的方法,用于选择性蛋白质修饰和净化,使用亲和力生物对等化学 (ABC) 标签.
为了证明修改后的蛋白质与转环氧烯 (TCO) 功能化的水凝微纤维的附着.
为了展示蛋白质功能维护和执行多蛋白质表面修饰的能力.

主要方法:

使用3 - - - - - - - - - - - - - - - - - - - 甲基四氨酸 (PyTz) 作为蛋白质修饰的亲和力生物直角化学 (ABC) 标签.
结合PyTz标记的蛋白质通过四链接到通过TCO功能化的水凝微纤维.
用各种特征的蛋白质验证了该方法:HaloTag,NanoLuc luciferase (NanoLuc) 和纤维素第3类域9-10 (FNIII 9-10).

主要成果:

证明了成功的选择性蛋白质结合,从而保持了蛋白质的功能.
在微纤维表面上展示了多种蛋白质 (HaloTag和NanoLuc) 的同时结合.
通过调整相对度 (HaloTag和超级文件 GFP [sfGFP]) 来说明多种蛋白质的受控呈现.

结论:

亲和力生物直角化学 (ABC) 标记方法为选择性蛋白质表面修饰提供了一种灵活和高效的方法.
这种方法适应不同大小和功能的蛋白质,保持它们的生物活性.
该技术可以精确控制单蛋白和多蛋白表面功能,扩大生物材料设计的可能性.

关键词:

3D细胞培养的3D细胞培养生物材料是一种生物材料.梯度梯度是指一个梯度.蛋白质蛋白质是蛋白质蛋白质的组成部分.脚手架的脚手架是一个脚手架.选择性地址选择性地址这是一种四氨酸.转环氧 octene 是一个转环氧 octene.

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