Ythdf2促进前体miR-378/miR-378-5p的成熟,以支持肌体分化
Kaiping Deng1,2, Yalong Su1,2, Zhipeng Liu1,2
1Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, 210095, China.
Cellular and molecular life sciences : CMLS
|November 6, 2024
概括
Ythdf2蛋白通过一种依赖m6A的机制控制特定微RNA (miRNA) 的成熟,从而调节肌肉细胞的分化,从而影响肌肉生成.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞生物学 细胞生物学
背景情况:
- Ythdf2蛋白调解mRNA降解,并影响骨肌肉的分化.
- Ythdf2与m6A修饰的前体miRNA相互作用,影响它们的成熟.
- 通过miRNA成熟,Ythdf2在肌体发生中的作用尚不清楚.
研究的目的:
- 为了调查Ythdf2是否通过控制miRNA成熟来调节肌体发生.
- 在肌体分化过程中识别Ythdf2准的特定miRNA.
- 阐明连接Ythdf2,miRNA成熟和肌体发生的分子机制.
主要方法:
- 在神经细胞中进行的Ythdf2敲击实验.
- 用于综合分析的miRNA和mRNA测序.
- 同免疫沉试验用于研究蛋白质相互作用.
- 西方涂抹和qRT-PCR用于评估蛋白质和miRNA的表达.
主要成果:
- Ythdf2倒置抑制了髓管的形成,改变了miRNA的表达.
- 确定了miR-378和miR-378-5p是肌肉形成中的关键Ythdf2标.
- 通过与DICER1和TARBP2相互作用,Ythdf2促进了miR-378/miR-378-5p前成熟.
- 降低miR-378/miR-378-5p的调节抑制了肌体发生;它们的强制表达通过mTOR通路拯救了Ythdf2的淘汰效应.
结论:
- Ythdf2通过介导前-miR-378/miR-378-5p成熟以一种m6A依赖的方式来调节肌原分化.
- 这种机制涉及Ythdf2与前-miRNA处理复合物的相互作用.
- 这些发现为m6A修饰在肌肉形成调节中的作用提供了新的见解.
相关概念视频
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Formation of Muscle Fibers from Myoblasts
4.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.8K
Satellite Stem Cells and Muscular Dystrophy
1.9K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
1.9K
Role of Hematopoietic Growth Factors
1.3K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Thrombopoietin (TPO), mainly released by the liver,...
1.3K
Functions of Thyroid Hormones
2.6K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.6K


