Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

4.8K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
4.8K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.4K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

1.1K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
1.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Differences in Salinity Stress Responses Across Developmental Stages and Tissue Regions in <i>Saccharina japonica</i>.

International journal of molecular sciences·2026
Same author

Research Progress of Grapefruit-derived Extracellular Vesicles in Anti-tumor Treatment.

Current medicinal chemistry·2026
Same author

Preparation and in vitro evaluation of photodynamic-responsive nanoliposome loaded PL-5.

PloS one·2026
Same author

Lipid droplets-mediated delivery of curcumin for targeted mitochondrial photodynamic therapy.

International journal of pharmaceutics: X·2026
Same author

Smartphone-Based Proactive Self-Screening for Ocular Surface Malignancies: A Nonrandomized Clinical Trial.

JAMA ophthalmology·2026
Same author

Optimal Dosage and Distribution of Bone Cement in Vertebroplasty: A Finite Element Analysis.

Clinical spine surgery·2026

相关实验视频

Updated: Jun 8, 2025

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
09:41

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

Published on: September 13, 2017

9.1K

解码胰岛素分泌颗粒成熟使用遗传编码的pH传感器.

Wen Lin1, Kaylee Tseng1, Scott E Fraser2

  • 1Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, California 90089, United States.

ACS sensors
|November 6, 2024
PubMed
概括

研究人员开发了新的工具来跟踪胰腺β细胞中的胰岛素成熟. 这项研究揭示了不同的胰岛素分泌颗粒群体以及信号如何控制它们的分泌,为糖尿病治疗提供了洞察力.

关键词:
飞行员的船只.它们是基因编码的.胰岛素分泌颗粒的颗粒.成熟路径的成熟路径一个pH值传感器.胰腺β细胞是什么

更多相关视频

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
05:58

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Published on: June 25, 2019

7.4K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K

相关实验视频

Last Updated: Jun 8, 2025

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
09:41

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

Published on: September 13, 2017

9.1K
Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
05:58

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Published on: June 25, 2019

7.4K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K

科学领域:

  • 细胞生物学 细胞生物学
  • 内分泌学 在内分泌学.
  • 生物化学 生物化学

背景情况:

  • 胰岛素是血糖调节的关键激素,通过酸化和酶分裂在分泌颗粒中成熟.
  • 胰岛素成熟失调与糖尿病有关,细胞控制机制尚不清楚.

研究的目的:

  • 开发用于高分辨率监测活细胞中胰岛素分泌颗粒 (ISG) pH动态的新工具.
  • 研究细胞信号如何影响ISG亚群和胰岛素成熟过程.

主要方法:

  • 设计和实施了基因编码的光pH传感器.
  • 建立了一个光终身成像和分析管道,用于精确的ISG pH监测.
  • 使用活细胞成像观察ISG行为和pH变化.

主要成果:

  • 根据pH值和亚细胞局部化确定了不同的ISG亚群.
  • 代谢信号和膜脱极化被证明可以调动不同的ISG亚群.
  • 成熟信号被证实诱导ISG酸化.

结论:

  • 细胞信号网络通过差异调节ISG的调动和分泌.
  • 开发的工具为研究ISG动态提供了前所未有的分辨率.
  • 这些发现为了解和治疗糖尿病提供了潜在的途径.