相关实验视频

Updated: Jul 23, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

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一种简单,具有成本效益和高效的方法来选植物中的CRISPR/Cas9突变物

Yiping Wang1, Jun Ma1, Yingying Wu1

  • 1College of Plant Protection, Yangzhou University, Yangzhou, Jiangsu, China.

Journal of plant physiology
|November 6, 2024
PubMed
概括

我们开发了一种新方法,PCR-Bsl I关联分析 (PCR-BAA),用于快速且负担得起的选聚类的定期间隔的短时间palindromic重复 (CRISPR) /CRISPR关联蛋白9 (Cas9) 在植物中的基因组编辑突变. 这种技术有效地识别突变物,即使是从低效率的编辑事件.

关键词:
阿拉比多普西斯 (Arabidopsis) 是一种植物.这就是为什么BSL.这就是CRISPR/Cas9的作用.突变查 突变查大米 大米 大米 大米 大米

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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins08:37

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CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...