相关实验视频

Updated: Jun 8, 2025

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

三桑丁通过MICU3介导的线粒体流入引起抗瘤活性

Yunbin Zhang¹, Xuping Ding², Qian Zhang²

¹Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Institute of Immunology, Shanghai JiaoTong University School of Medicine; Academy of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Journal of advanced research

|November 6, 2024

概括

三桑丁 (TK) 通过向线粒体通道MICU3来抑制肺腺癌的生长. 这种糖蛋白抑制DNMT3B并增加FOSL2,导致瘤细胞死亡和迁移减少.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

三桑丁 (TK) 是一种来自Trichosanthes kirilowi的糖蛋白,具有抗病毒和抗瘤的特性.
确切的分子标和基底的机制TK的抗瘤活性仍然在很大程度上未被阐明.

研究的目的:

为了确定TK在肺腺癌中的新型抗瘤点.
阐明TK在肺腺癌中的详细抗瘤机制.

主要方法:

使用易斯肺癌小鼠模型的体内研究和在A549和H1299细胞系的体内测试.
通过RNA测序分析线粒体水平,膜潜力和转录组变化的分析.
通过使用qPCR,Western blotting,ChIP-PCR和光酶试验,研究DNMT3B表达,MICU3甲基化和转录活性.

主要成果:

在体内,TK显著抑制瘤生长,诱导癌细胞死亡,对正常细胞的影响最小.
通过TK对MICU3的上调与瘤细胞生长和迁移的减少以及细胞死亡的增加以及线粒体水平的升高相关.
TK治疗改变了DNMT3B和MICU3甲基化,同时增加了FOSL2表达,从而促进了MICU3基因转录.

结论:

三桑丁通过调节DNMT3B和FOSL2表达,在肺腺癌中表现出抗瘤活性.
这种调节导致诱导由MICU3介导的线粒体流入,最终导致瘤细胞死亡.

关键词:

在DNMT3B中,它是DNMT3B.这就是FOSL2的原因.肺部腺癌瘤是肺部腺癌.在MICU3中使用MICU3.三桑丁是一种三桑丁.

更多相关视频

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

相关概念视频

Drugs that Stabilize Microtubules

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...