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甜桃HIPPs的分子识别和表达模式以及PavHIPP16在寒冷应力中的功能分析

Runrun Yu¹, Qiandong Hou¹, Hong Deng¹

¹Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.

|November 6, 2024

概括

与重金属相关的异烯化植物蛋白 (HIPPs) 调节了甜桃的压力反应. PavHIPP16增强了植物的抗寒能力,为水果作物提供了基因改进的潜力.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
压力生理学压力生理学

背景情况:

与重金属相关的异烯化植物蛋白 (HIPPs) 对植物非生物应激反应至关重要.
在甜桃 (Prunus avium L.) 中对HIPP的系统研究是有限的.

研究的目的:

在甜桃中进行PavHIPP基因的综合分析和表达概况.
调查PavHIPP16在耐寒压力中的作用.

主要方法:

生物信息学分析
用于表达形状的RT-PCR和qRT-PCR进行表达形状.
阿拉比多普西斯塔利亚纳过度表达研究

主要成果:

鉴定了28个分布在八个染色体上的PavHIPP基因.
在PavHIPP的推广区域中,包含了与压力相关的监管要素.
在Arabidopsis中过度表达PavHIPP16增强了寒冷耐受性,通过降低导电性和MDA,以及增加的プロ林,POD和SOD活性来表明.

结论:

PavHIPP基因参与低温压力和甜桃的发展.
在改善抗寒性方面,PavHIPP16起着重要作用.
PavHIPP基因是甜桃基因改进的潜在目标,以提高应激适应能力.

关键词:

寒冷的压力是冷的压力.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式功能分析是一种功能分析.基因家族基因家族基因转换是一种基因转换.与重金属相关的异烯酸植物蛋白.

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