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相关概念视频

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

4.5K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
4.5K
ATP Synthase: Structure01:18

ATP Synthase: Structure

11.9K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
11.9K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

3.6K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.6K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.0K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.0K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

13.9K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
13.9K
The ADP/ATP Carrier Protein01:42

The ADP/ATP Carrier Protein

3.2K
ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
3.2K

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相关实验视频

Updated: Jun 8, 2025

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
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Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Published on: January 22, 2019

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P5A-ATPases的结构和功能

Ping Li1, Viktoria Bågenholm2, Per Hägglund2

  • 1Department of Experimental Medical Science, Lund University, Sölvegatan 19, SE-221 84, Lund, Sweden. ping.li@med.lu.se.

Nature communications
|November 6, 2024
PubMed
概括

P5A-ATPases对于内质网中的蛋白质质量控制至关重要. 这项研究揭示了它们的运输机制,展示了它们如何通过膜结合和移动蛋白质载荷,这可能有助于蛋白质的移除或插入.

更多相关视频

Measuring In Vitro ATPase Activity for Enzymatic Characterization
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Measuring In Vitro ATPase Activity for Enzymatic Characterization

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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

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相关实验视频

Last Updated: Jun 8, 2025

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
08:44

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Published on: January 22, 2019

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Measuring In Vitro ATPase Activity for Enzymatic Characterization
07:38

Measuring In Vitro ATPase Activity for Enzymatic Characterization

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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
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Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

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科学领域:

  • 结构生物学 结构生物学
  • 分子细胞生物学 分子细胞生物学
  • 生物化学 生物化学

背景情况:

  • 细胞内网 (ER) 膜中居住的P5A-ATPases在蛋白质生物发生和质量控制中发挥着广泛的作用.
  • 对于P5A-ATPases的精确分子功能仍然不完全理解.
  • 了解这些ATPase对于破译细胞蛋白质管理途径至关重要.

研究的目的:

  • 通过确定它们的结构动态来阐明P5A-ATPases的分子机制.
  • 在高分辨率下可视化P5A-ATPase,CtSpf1的传输周期.
  • 识别潜在的蛋白质载荷和特定结构域的作用.

主要方法:

  • 使用冷电子显微镜 (cryo-EM) 来捕捉多个结构状态.
  • 确定了CtSpf1 ATPase催化循环 (E1到E2状态) 的各种中间体的结构.
  • 分析的重点是形状变化,基质结合点和域移动.

主要成果:

  • 低温电磁结构显示了CtSpf1在整个催化周期中的不同构造.
  • 在E2P和E2.Pi状态下,观察到一个跨膜裂,结合一个多载荷.
  • E1状态结构显示了一个面向细胞质的腔,被一个"Plug-domain"遮住,在后来的状态中被取代.

结论:

  • P5A-ATPases可能会结合各种各样的蛋白质载体.
  • 观察到的结构特征表明它在去除跨膜螺旋体方面发挥了作用,并可能插入或分泌.
  • 插件域似乎在ATPase功能和基质转位中发挥着关键的机械作用.