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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jun 8, 2025

Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments
11:47

Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments

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使用微球辅助白光干扰纳米镜进行反射映射.

Sébastien Marbach1, Rémy Claveau2, Paul Montgomery2

  • 1ICube, Université de Strasbourg, CNRS, INSA, 67000, Strasbourg, France. sebastien.marbach@etu.unistra.fr.

Scientific reports
|November 6, 2024
PubMed
概括
此摘要是机器生成的。

一种新的微球辅助纳米镜增强了白光干扰显微镜用于纳米级材料表征. 这种技术可以提高横向分辨率,用于非破坏性,无标签的局部反射率映射.

关键词:
局部光谱学 局部光谱学微球微球是指微球的小部分.纳米显微镜使用的方法.反射力 反射力是一种反射力.决议 决议 决议白光干扰显微镜技术

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Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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Implementation of a Reference Interferometer for Nanodetection
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Implementation of a Reference Interferometer for Nanodetection

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相关实验视频

Last Updated: Jun 8, 2025

Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments
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Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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科学领域:

  • 材料科学 材料科学 材料科学
  • 光学计量学 在光学计量学
  • 纳米技术纳米技术

背景情况:

  • 在纳米尺度上对新型材料进行表征具有重大挑战.
  • 现有的技术可能缺乏某些测量所需的分辨率或侵入性.

研究的目的:

  • 介绍一种新的微球辅助白光干扰纳米镜,用于纳米尺度局部反射率映射.
  • 为了证明材料表征的侧向分辨率得到了改善.

主要方法:

  • 在白光干扰显微镜中使用了一个直径为145微米的微球,连接到光纤上.
  • 采用里叶变换处理干扰图像堆来提取局部反射率.
  • 在波长范围为460-900nm的波长范围内运行,视野为8μm.

主要成果:

  • 从940nm到520nm实现了增强的横向分辨率.
  • 在一个以激光构造的不钢样本上,成功地在光谱上区分了相邻的波纹,距离279nm.
  • 证明了非破坏性,全场和无标签的局部反射映射.

结论:

  • 微球辅助纳米镜显著提高了用于反射率映射的横向分辨率.
  • 这种技术使得材料表面的纳米级特征能够得到详细的描述.
  • 未来的工作目标是以100nm分辨率进行次衍射反射率映射.