针对感染的特定人类基因表达是诊断潜伏和活跃结核病的有效标志物
Ritah Nakiboneka1,2,3,4, Natasha Walbaum1, Emmanuel Musisi1,5
1Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, KY16 9TF, UK.
Scientific reports
|November 6, 2024
概括
一种新的RT-qPCR试验可以使用宿主基因标记来诊断活性肺结核 (TB) 和潜伏性结核感染 (LTBI). 这种近临床检测可以将结核病与其他呼吸道疾病和健康个体区分开来.
科学领域:
- 分子生物学分子生物学
- 传染病诊断 传染病诊断 传染病诊断
- 宿主-病原体相互作用
背景情况:
- 全血中的转录标记可以区分活跃肺结核 (ATB) 与其他呼吸道疾病 (ORD) 以及潜伏结核感染 (LTBI) 与健康对照 (HC).
- 现有的诊断方法可能缺乏广泛应用所需的速度或可访问性.
研究的目的:
- 开发和评估一种简化的逆转录酶定量聚合酶链反应 (RT-qPCR) 试验,用于诊断ATB和LTBI.
- 区分ATB与ORD和LTBI与HC使用宿主基因标记物 (HGM) 在近照顾点设置中.
主要方法:
- 文献审查以确定结核病感染的可信的HGM.
- 设计和对选定HGM的原料和探针进行体外分析评估.
- 将表现最好的基因复合成组,用于临床评估.
- 与Mycobacteria生长指标管和QuantiFERON-TB Gold Plus作为参考标准进行比较.
主要成果:
- 十六个HGM被纳入了五个多重RT-qPCR面板,具有高PCR效率 (≥90%).
- 临床评估显示了不同的基因表达模式:ATB与ORD相比较高,LTBI与HC相比较低 (p < 0.0001).
- 特定的HGM (例如,BATF2,CD64,GBP5) 显示出高度区分ATB的能力,而其他 (例如,KLF2,PTPRC) 则歧视LTBI.
结论:
- 基于宿主基因的RT-qPCR试验可用于诊断ATB和LTBI在临床附近.
- 为了区分ATB和LTBI与控制,可能需要不同的HGM集.
- 开发的试验显示了改善结核病诊断的潜力.
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