德克萨米他是丸周管细胞中时钟基因的调节者
Harald Welter1, Nicole Kreitmair1, Michaela Schneider1
1Biomedical Center, Cell Biology, Anatomy III, Faculty of Medicine, Ludwig Maximilian University of Munich, Planegg-Martinsried, Munich, Germany.
Andrology
|November 7, 2024
概括
葡萄糖皮质醇 (GC) 调节丸周管细胞中的内部时钟. 德克萨米他 (Dex) 激活葡萄糖皮质体受体 (GR),显著增加这些细胞中的时钟基因表达,表明GC.
科学领域:
- 生殖生物学和内分泌学.
- 时间生物学 时间生物学
- 分子细胞生物学分子细胞生物学
背景情况:
- 人体丸中的周周细胞表达葡萄糖皮质体受体 (GR).
- 德克萨米他 (Dex) 的GR激活严重改变丸周管细胞表型,蛋白质组和分泌组.
- 葡萄糖皮质体 (GC) 是已知的调节者在周围器官的昼夜钟基因.
研究的目的:
- 为了研究德克斯对人类丸周管细胞 (HTPC) 时钟基因表达的影响.
- 为了确定丸周管细胞是否具有内源的昼夜节律.
- 探索丸GR在调节丸时钟中的作用.
主要方法:
- 使用HTPC和非人类灵长类动物 (Callithrix jacchus; Cj) 永久化的周管细胞进行的细胞研究.
- Cj丸碎片的器官类型化.
- 定量PCR (qPCR),蛋白质组分析和免疫组织化学.
主要成果:
- 在HTPC中,基底时钟基因表达表现出24小时的变化,表明内生节律.
- 德克斯治疗显著增加了HTPC和Cj周管细胞中的PER1和BMAL1mRNA水平.
- 德克斯还在Cj丸碎片的器官类型培养物中增加了BMAL1和PER1mRNA.
结论:
- 丸周管细胞可能具有由GC通过丸GR调节的内部时钟系统.
- 在生理条件下,GC可能是丸钟的关键调节者.
- 在长时间的压力或GC药物治疗期间,丸钟基因表达的干扰可能会发生.
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