VBNC Cronobacter sakazakii通过抵抗氧化应激并逃避巨细胞的识别而在巨细胞中生存
Yuanyuan Liu1, Jingfeng Zhang1, Haoqing Zhao1
1Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Sciences, South China Agricultural University, Guangzhou, Guangdong Province, 510642, China.
BMC microbiology
|November 7, 2024
概括
可存活但不能培养的Cronobacter sakazakii (C. sakazakii) 通过加强通过严格反应的氧化耐受性,并通过改变的脂多糖 (LPS) 合成逃避免疫识别,从而在巨细胞的防御中存活下来,从而构成致病风险.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 细菌病原体的产生
背景情况:
- 致病细菌利用宿主巨细胞生存和致病.
- 可存活但不能培养的 (VBNC) Cronobacter sakazakii (C. sakazakii) 可以在巨细胞中存活,但潜在的机制尚不清楚.
研究的目的:
- 为了研究巨细胞内VBNC C. sakazakii的生存机制.
- 为了探索 VBNC C. sakazakii 的环境耐受性和逃避巨细胞识别.
主要方法:
- 在氧化应激下对VBNC和可培养的C. sakazakii存活率进行比较分析.
- 严格反应和抗氧化剂相关基因的基因表达分析.
- 评估巨细胞的托尔类受体4 (TLR4) 反应和脂多糖 (LPS) 合成.
- 对酸对细菌生存和巨细胞免疫识别的影响的评估.
主要成果:
- 与可培养形式相比,VBNC C. sakazakii在氧化条件下表现出更高的生存率.
- 在VBNC C. sakazakii.中观察到严格反应 (relA, spoT) 和抗氧化基因 (sodA, katG, trxA) 的升级.
- VBNC C. sakazakii诱导了巨细胞中减少的TLR4反应,与抑制的LPS生物合成有关.
- 埃拉基酸通过增强巨细胞TLR4识别来降低细菌的存活率.
结论:
- VBNC C. sakazakii通过严格的反应来调节氧化耐受性,从而在巨细胞环境中生存.
- 在VBNC中改变LPS合成的C. sakazakii有助于逃避TLR4-介导的巨细胞识别.
- 这些发现突显了VBNC C. sakazakii的致病潜力,并提出了治疗点.
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