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来自Populus tomentosa的PtoCYP90D1基因与铜类生物合成相关的分子克隆和特征

Juntao Song¹, Jie Tan¹, Tao Long¹

¹College of Tobacco Sciences, Guizhou University, Guiyang, 550025, China.

|November 7, 2024

概括

铜类基因PtoCYP90D1增强了树的生长和生物量. 过度表达增加了植物的高度和茎直径,而突变减少了生物质,突出了它在植物发育中的积极作用.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

铜类固醇 (BRs) 是关键的植物激素,调节植物生长,发育和应激反应.
在阿拉比多普西斯中的AtCYP90D1是一种细胞染色体P450单氧化酶,参与了布拉西诺利德生物合成.
人类毛虫 (Populus tomentosa) 拥有同类基因的AtCYP90D1,在生长和发育中可能发挥作用.

研究的目的:

为了功能性地描述PtoCYP90D1,这是来自Populus tomentosa的AtCYP90D1的同类.
调查PtoCYP90D1在调节生长,生物质生产和细胞壁组件中的作用.

主要方法:

定量实时PCR (qRT-PCR) 用于分析PtoCYP90D1表达模式.
在树中使用过度表达 (PtoCYP90D1-OE) 和CRISPR/Cas9淘汰 (PtoCYP90D1-KO) 来对PtoCYP90D1进行基因操纵.
转基因树系的表型分析,包括测量植物高度,茎直径,生物质产量,树皮面积和细胞壁组成部分.

主要成果:

在树根和老叶中,PtoCYP90D1的表达高.
过度表达PtoCYP90D1显著增加了植物的高度,茎直径,生物质产量,树皮面积和细胞层.
淘汰PtoCYP90D1导致生物质生产显著下降.
与野生型和PtoCYP90D1-KO线相比,PtoCYP90D1-OE线的细胞壁成分显著增加.

结论:

PtoCYP90D1在促进树的生长速度和增强生物质生产方面发挥着积极作用.
这些发现表明PtoCYP90D1是影响发育的布拉西诺固醇路径的关键调节者.
PtoCYP90D1在改善树的工业和农业用途方面具有潜在的应用.

关键词:

在CYP90D1中.这种植物名为Populus tomentosa.生物质是生物质中的一部分.黄铜固醇是一种黄铜固醇.二次细胞壁中的二次细胞壁.

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