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一步,快速,基于纳米粒子的生物传感器平台,用于在临床应用中同时识别B型肝炎病毒和C型肝炎病毒

Xu Chen^1,2, Yuanfang Shi¹, Qi Zhao³

¹The Second Clinical Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550003, People's Republic of China.

BMC microbiology

|November 7, 2024

概括

一种新的多重分子诊断试验 (mLAMP-AuNPs-LFB) 提供了对乙型肝炎 (HBV) 和C型肝炎 (HCV) 病毒的快速,敏感和特定的治疗点检测. 这种用户友好的工具显示出全球公共卫生的巨大潜力,特别是在资源有限的环境中.

科学领域:

分子诊断学分子诊断学
生物技术是生物技术.
公共卫生公共卫生

背景情况:

由HBV和HCV引起的病毒性肝炎对全球健康构成重大挑战,特别是在低收入和中等收入国家.
有效的查和诊断需要敏感,具体,快速和用户友好的 Point-of-Care (POC) 测试平台.

研究的目的:

开发和验证一种用于同时识别HBV和HCV的新型分子诊断试验.
建立一种用户友好,快速和敏感的POC病毒肝炎检测方法.

主要方法:

开发一个多重循环介导的同热放大 (mLAMP) 试验,与基于金纳米粒子的侧流生物传感器 (AuNPs-LFB) 集成.
针对HBV S基因和HCV 5'-UTR基因的特定原始体的设计,优化了放大温度和时间.
使用临床样本验证试验灵敏度,特异性和临床可行性.

主要成果:

成功制造了AuNPs-LFB,并设计了用于HBV和HCV检测的独特的原始配对.
完整的mLAMP-AuNPs-LFB测定,包括核酸分离,mLAMP和视觉解释,可以在50分钟内完成.
该试验表现出高灵敏度,每次检测只能检测到20个目标基因副本,对实验性病原体具有100%的特异性.

结论:

开发的mLAMP-AuNPs-LFB测定是HBV和HCV鉴定的一个有价值的工具.
这种测试具有作为临床检测方法的巨大潜力,特别是在服务不足的地区.
该测试的用户友好和快速性质支持其在全球公共卫生倡议中的应用,以控制病毒性肝炎.

关键词:

生物传感器是一种生物传感器.乙型肝炎病毒的感染.肝炎C病毒感染者循环介导的同热放大方式.护理点平台的平台.

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