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Updated: Jun 8, 2025

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

在传染过程中重新排列光检测的重新排列在NSCLC中与其他技术相比,在现场混合化

Anne Mc Leer^1,2,3, Julie Mondet^1,2,3, Nelly Magnat²

¹Université Grenoble Alpes, Grenoble, France.

JTO clinical and research reports

|November 7, 2024

概括

在非小细胞肺癌 (NSCLC) 中,使用光在位杂交 (FISH) 选RET重组. 虽然FISH是一种有用的查工具,但RNA测序确认对于准确选择患者接受RET向治疗至关重要.

科学领域:

在瘤学瘤学.
分子诊断学分子诊断
遗传学是一个遗传学.

背景情况:

在非小细胞肺癌 (NSCLC) 的1-2%中发生RET重组.
没有临床验证的RET抗体可用,使得光在位杂交 (FISH) 成为常见的查方法.
准确识别RET重组对于指导RET向治疗至关重要.

研究的目的:

对RET-FISH测试的现有文献进行审查和比较,并与内部数据进行比较.
评估RET-FISH在NSCLC中识别RET重组的疗效和一致性.
评估对RET-FISH阳性病例进行直角确认的必要性.

主要方法:

对使用RET-FISH进行重排测试的研究进行了全面的文献审查.
分析了格勒诺布尔大学医院的数据,包括784个EGFR-, KRAS-, ALK-和ROS1-负的NSCLC通过RET分离FISH进行测试.
用RNA测序 (RNA-seq) 来确认RET-FISH的结果.

主要成果:

在85个出版物中,52个专注于肺癌,其中15%是RET-FISH最常见的阳性门.
在已发表的研究中,RET-FISH与其他分子技术之间的一致性差异很大 (5.9%至66.7%).
内部数据显示,4% (32/784) 的NSCLC样本是RET-FISH阳性的,RET-FISH和RNA-seq.之间有69%的一致性.

结论:

在NSCLC中,RET-FISH是快速选RET重组的一个有价值的工具.
使用RNA-seq等技术进行正对角确认对于确保准确选择患者接受RET向疗法至关重要.
将RET-FISH查与RNA-seq确认相结合,可以优化针对性治疗的患者分层.

关键词:

鱼类鱼类是一种鱼类.融合基因是一个融合基因.肺癌是一种肺癌.在RET的基础上,RET是RET.通过RNA测序来测序RNA序列.有针对性的治疗.

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相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...