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Updated: Jun 8, 2025

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
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缺氧和TNF-α调节了人类诱导的多能干细胞衍生心肌细胞的细胞外囊释放

Margarida Viola1, Maarten P Bebelman2, Renee G C Maas1

  • 1Department of Experimental Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.

Journal of extracellular vesicles
|November 7, 2024
PubMed
概括

心肌细胞衍生的细胞外囊泡 (EVs) 对于心脏通信至关重要. 这项研究揭示了营养缺乏,缺氧和炎症如何调节心肌细胞中的EV产生,为心脏病提供了新的见解.

关键词:
在EV生物发生过程中,EV生物生成在NanoLuc中使用NanoLuc.这是心肌细胞 (cardiomyocytes).细胞外囊泡中的细胞外囊泡.心肌梗塞的心脏病发作

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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