用于超声波触发炎性肠病治疗的DNA纳米包特异性修饰益生菌
Xiangbowen Jin1, Hongyang Li1, Sheng Pan2
1Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano & Soft Materials & Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University, Suzhou 215123, China.
用DNA纳米补丁制造的益生菌对炎症性肠道疾病有好处. 这种系统通过提供抗炎细胞因子和抵抗氧化应激来增强肠道屏障功能并减少炎症.
科学领域:
- 生物技术
- 纳米技术
- 微生物学
背景情况:
- 益生菌具有治疗炎症性肠病 (IBD) 的潜力.
- 挑战包括精确控制和对益生菌的抗氧化应激.
- 工程化益生菌为克服这些局限性提供了一条道路.
研究的目的:
- 开发超声波触发的工程益生菌,增强氧化应激抵抗力和有针对性的输送.
- 提高益生菌在胃肠道中的生物可用性和有效性,用于IBD治疗.
主要方法:
- 合成细菌特异性DNA纳米斑 (DNP) 用于修改工程化大肠杆菌Nissle 1917.
- DNP通过马尔托德清除反应性氧物种并向细菌.
- 使用酸盐和酸盐进行封装的工程益生菌,以提高生物可用性.
主要成果:
- 经过超声波刺激,工程化益生菌产生了互白素-10.
- DNP选择性地附着在细菌表面,而不是哺乳动物细胞.
- 在小鼠结肠炎模型中,DNP-益生菌系统显著改善了肠道屏障的完整性,并减少了炎症.
结论:
- DNA纳米贴-细菌系统有效地减轻氧化应激,并纠正微生物失调.
- 这种方法增强了肠道屏障功能,
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