相关实验视频

Updated: Jun 8, 2025

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

多功能转录因子YABBY6调节大米的形态发生,干旱和寒冷应激反应

Jia Zuo¹, Cuijie Wei¹, Xiaozhu Liu²

¹School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China.

Rice (New York, N.Y.)

|November 7, 2024

概括

米基因OsYABBY6影响植物结构和谷粒大小. 它增强了寒冷耐受性,但通过调节压力反应和酸信号传递来改善干旱耐受性.

科学领域:

植物分子生物学植物分子生物学
遗传学是一个遗传学.
生物化学生化学

背景情况:

YABBY转录因子家族在植物发育中发挥着多种作用.
在大米中OsYABBY6的特定功能在很大程度上仍未被描述.

研究的目的:

为了阐明大米YABBY基因的功能,OsYABBY6.6.
研究其在植物结构,谷物发育和非生物应激反应中的作用.

主要方法:

描述OsYABBY6局部化和转录活动.
在大米组织中分析OsYABBY6表达模式.
产生和分析OsYABBY6RNA干扰 (RNAi) 和过度表达 (OE) 植物.
评估植物对寒冷和干旱压力的反应,包括生理和分子分析.
对酸 (ABA) 敏感性的评估.

主要成果:

OsYABBY6是一种核局部蛋白质,具有转录激活活性,主要表达在花器官和植物组织中.
OsYABBY6 RNAi植物的植物高度发生了变化,颗粒大小增加.
OsYABBY6 OE植物表现出增强的寒冷耐受性,高调节的寒冷反应基因和减少的活性氧物种 (ROS).
OsYABBY6 RNAi植物表现出改善的干旱耐受性,其特点是水损失较慢,ROS积累减少.
OsYABBY6影响酸 (ABA) 水平和灵敏度,对干旱反应产生负面调节,对寒冷反应产生积极调节.

结论:

OsYABBY6是大米植物结构和谷物发育的关键调节剂.
OsYABBY6在非生物性压力中起着双重作用,积极调节耐寒性,负面调节耐干旱性.
这项研究为YABBY基因在米发育和应激适应中的多方面的功能提供了新的见解.

关键词:

俄斯亚巴比 6 的意思阿巴阿巴阿巴是什么意思寒冷的寒冷的寒冷的寒冷的寒冷的寒冷干旱干旱干旱干旱粒度大小粒度大小粒度大小植物建筑植物建筑大米大米大米大米大米

更多相关视频

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

相关概念视频

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...