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封闭输液超视网膜ERG设置用于临床前药物和纳米结构测试.

Sama Saeid, Marja Pitkanen, Emma Ilonen

    IEEE transactions on bio-medical engineering
    |November 7, 2024
    PubMed
    概括

    一个新的封闭 perfusion 超视网膜电网膜学 (tERG) 设置允许长期的临床前药物测试使用最小的 perfusate. 这种方法表明,测试的纳米结构材料对视网膜细胞没有不良影响.

    科学领域:

    • 神经科学是一个神经科学.
    • 药理学 药理学是指药理学的学科.
    • 生物医学工程 生物医学工程

    背景情况:

    • 孤立的哺乳动物视网膜是用于临床前药物评估的潜在生物传感器.
    • 在长时间的测试期间,在有限的 perfusate 中保持视网膜活力存在挑战.

    研究的目的:

    • 开发一个封闭 perfusion 超视网膜电视图 (tERG) 设置,用于高效的临床前药物测试.
    • 以最小的药物分子或纳米结构进行测试.

    主要方法:

    • 在老鼠视网膜上进行了ex vivo tERG (WT C57BL/6J).
    • 视网膜被以5-6毫升/分钟的透率在需要~50毫升透剂的封闭循环系统中 perfused.
    • 使用闪光灯评估了棒和圆反应,长时间记录使用了青素-杆菌素.

    主要成果:

    • 棒的反应保持稳定超过42小时,棒/圆双极细胞的反应长达12小时.
    • 该设置使用IBMX (一种PDE抑制剂) 进行了验证.
    • 测试材料,聚硫甲酸 (PSMBA) 和其单体SBMA,没有对杆状和双极细胞功能产生不良影响.

    结论:

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    • 开发的封闭 perfusion tERG 设置可方便使用最小 perfusate 的扩展实验.
    • 该方法适用于新型分子和纳米结构的药物功能,生物相容性和毒性评估.