梅塔希齐乌姆战斗俱乐部:主机内部的竞争排斥和资源分割
Huiyu Sheng1, Raymond J St Leger1
1Department of Entomology, University of Maryland, College Park, Maryland, United States of America.
PLoS pathogens
|November 7, 2024
概括
根据宿主和营养条件,Metarhizium robertsii (Mr2575) 和Metarhizium anisopliae (Ma549) 菌株的竞争方式不同. Mr2575在营养较差的环境中表现出色,而Ma549则在营养较差的环境中表现出色.
科学领域:
- 菌类学和微生物生态学
- 昆虫病理学 昆虫病理学
- 植物与微生物的相互作用
背景情况:
- Metarhizium物种是具有生态系统和农业作用的虫病原菌.
- 了解菌株竞争对于它们的有效应用至关重要.
- 对影响Metarhizium菌株之间竞争能力的因素的知识有限.
研究的目的:
- 为了研究Metarhizium robertsii ARSEF 2575 (Mr2575) 和Metarhizium anisopliae ARSEF 549 (Ma549) 之间的竞争相互作用.
- 确定环境条件和宿主因素如何影响这两种Metarhizium菌株的竞争成功.
- 阐明共感染期间菌株分离和资源分离的基本机制.
主要方法:
- 在营养限制条件下进行体外竞争测试.
- 在Arabidopsis thaliana,Manduca sexta和Drosophila melanogaster的同时感染实验.
- 分析胚芽菌的产生,菌体的形成和宿主内部的空间分布.
- 评估宿主反应,如黑色化和对缺氧的易感性.
主要成果:
- Mr2575在营养有限的条件下和在Arabidopsis根上超过了Ma549的竞争.
- 在较大的昆虫 (M. sexta) 中,Ma549的更高的胚芽菌生产赋予了竞争优势,有助于分散和抑制宿主反应.
- 在宿主中观察到菌株分离,Mr2575在Drosophila胸部和前部M. sexta段中占主导地位,Ma549在M. sexta腹部和后部段中占主导地位.
- 在M. sexta hemocoel中,状菌的均分散使竞争能力相提并论,突显了感染路线的重要性.
结论:
- Metarhizium菌株的竞争能力取决于环境,受营养的可用性,宿主大小和感染策略的影响.
- 胚芽体的产生是Ma549在较大的宿主中竞争力的关键因素,但随着均的体分散,它的优势会减少.
- 在昆虫宿主内部发生了资源分割,这表明Mr2575和Ma549.5之间的利基专业化.
- 这些发现对于理解Metarhizium的生态作用和优化其在生物控制和农业中的使用至关重要.
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