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重复基因驱动元件的中介切除,以恢复野生类型种群

Pratima R Chennuri¹, Josef Zapletal², Raquel D Monfardini¹

¹Department of Entomology and AgriLife Research, Texas A&M University, College Station, Texas, United States of America.

|November 7, 2024

概括

一个驱动元件的重复中介切除 (ReMEDE) 使用单链化用于精确的基因驱动器去除. 这项技术显示了在没有环境修改的情况下恢复野生类型种群的潜力.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
基因驱动技术基因驱动技术

背景情况:

基因驱动系统可以迅速改变野生种群.
开发安全和可逆的基因驱动系统对于生态应用至关重要.
需要缓解策略来控制或逆转意想不到的基因驱动效应.

研究的目的:

开发和评估作为基因驱动减轻策略的驱动元件重复中介切除 (ReMEDE).
通过单链化 (SSA) 来证明驱动元件的精确自催化切除.
评估ReMEDE在Drosophila melanogaster中用野生类型的等位基因替换驱动等位基因的疗效.

主要方法:

工程直向重复并排一个驱动基因.
在驱动等位基因内诱导双链DNA断裂 (DSB).
将ReMEDE纳入一种变性连锁反应 (MCR) 基因驱动器,准黄色基因.
通过测序分析基因转换和遗传模式.

主要成果:

在Drosophila melanogaster中,ReMEDE成功地用野生类型的等位基替换了驱动等位基.
测序证实了SSA的转基因切除,默默标记的遗传率为~4%.
观察到的是具有保留或突变序列的表型野生类型的,以及~14%完整的父继承等位基因.

结论:

通过使驱动元件的精确自催化切除,ReMEDE展示了作为基因驱动减轻策略的潜力.
该技术可以在没有额外的转基因释放的情况下恢复野生类型的种群.
需要进一步的研究来优化ReMEDE,以作为基因驱动逆转工具的更广泛应用.

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