通过植物和细菌TIR免疫信号激活辅助NLR
Hua Yu1,2, Weiying Xu1,2, Sisi Chen1,2
1Key Laboratory of Plant Design, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
概括
植物免疫受体使用Toll/interleukin-1受体 (TIR) 域来检测病原体. 这项研究揭示了EDS1-PAD4-ADR1 (EPA) 综合体如何发出信号,
科学领域:
- 植物免疫学
- 分子生物学
- 生物化学
背景情况:
- 植物细胞内核酸结合丰富的重复受体 (NLR),具有N-终端Toll/interleukin-1受体 (TIR) 域,对于检测病原体作用因子和启动免疫信号通路至关重要.
- TIR 域具有 NADase 活性,产生像基氨酸单酸/二酸 (pRib-AMP/ADP) 或循环 ADPR (cADPR) 异构体这样的信号分子,这些异构体在不同的王国中保存.
- 类似脂酶的蛋白质EDS1和PAD4作为信号传感器,将来自传感器TIR-NLR的信息传递到辅助NLRADR1,该NLR执行植物的免疫反应.
研究的目的:
- 阐明阿拉比多普西斯EDS1-PAD4-ADR1 (EPA) 异构体的结构和功能.
- 研究pRib-AMP/ADP及其相关分子在植物和细菌TIR信号的EPA复合体激活中的作用.
- 探索2'cADPR作为植物免疫中的信号分子的潜在作用.
主要方法:
- 阿拉比多普西斯 (Arabidopsis EPA) 的结构分析.
- 生物化学测定以确定EPA与pRib-AMP/ADP的相互作用.
- 涉及植物和细菌TIR信号通路的功能研究,以评估EPA激活.
主要成果:
- 该研究报告了与pRib-AMP/ADP复合的Arabidopsis EPA异构体的结构.
- 通过植物或细菌TIR信号产生的pRib-AMP/ADP激活了EPA信号.
- 发现2'cADPR被水解成pRib-AMP,随后激活EPA信号,产生2'cADPR的细菌TIR域也激活了EPA功能.
结论:
- EDS1-PAD4-ADR1 (EPA) 综合体在植物免疫信号传递中发挥着核心作用.
- 这些发现表明,2'cADPR可能作为短暂信号分子pRib-AMP的稳定存储形式.
- 这项研究提供了TIR-NLR调解植物免疫反应的分子机制的新见解.
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