在NTS中,CCK表达神经元由CCK敏感的C型阴道 afferents直接激活
Eric T Winzenried1, Drew M Neyens1, Rowan Calkins1
1Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, Washington, United States.
概括
孤独通道 (NTS) 中核胆囊托基宁 (CCK) 神经元是直接被阴道 afferents 激活的. 这些神经元整合肠道信号关于食物含量和大小,影响食物摄入控制.
科学领域:
- 神经科学是一个神经科学.
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- 阴道感官附带体将胃肠道 (GI) 信号传输到孤独通道 (NTS) 的核心.
- 胆囊托基宁 (CCK) 是一种和激活阴道 afferents,但其在NTS中的作用是不太了解.
- 众所周知,NTS CCK神经元会降低食物摄入量,但它们被 afferents激活的机制尚不清楚.
研究的目的:
- 为了研究阴道 afferents 如何激活 NTS CCK 神经元.
- 确定NTS CCK神经元所集成的关于食大小和内容的信息类型.
- 阐明CCK和血清素敏感的阴道 afferents对NTSCCK神经元激活的贡献.
主要方法:
- 使用CCK-IRES-Cre和floxed-Rosa-td番茄小鼠来识别NTSCCK神经元.
- 在水平脑切片中从NTS CCK神经元进行全细胞电压记录.
- 在孤独通道 (ST) 中刺激了阴道 afferents,并分析了刺激后突触电流 (EPSCs).
- 用CCK,5-基胺3受体 (5-HT3R) 激动剂和素来评估神经元敏感性.
主要成果:
- 80%的NTSCCK神经元接收直接的单突触输入从阴道 afferents.
- NTS CCK神经元通过AMPA和NMDA受体被谷氨酸激活.
- 这些神经元是素敏感C纤维的下游.
- CCK和5-HT3R激动剂在NTSCCK神经元的一个子集中增加了自发EPSC频率 (69%对CCK敏感,42%对5-HT3R敏感).
结论:
- NTS CCK神经元由酸盐直接从阴道 afferents 激活.
- 这些神经元主要接收来自CCK敏感的阴道 afferents的输入,以及一些来自血清素敏感的 afferents的输入.
- NTS CCK神经元整合与脂肪,蛋白质和胃伸展相关的胃肠道信号,并将这些信息传递给食物摄入调节.
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