Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Proximity-Based Phospho-Interactome (Prob-PhI) Characterization Reveals Distinct Signaling Activities of MEK1 and MEK2.

Analytical chemistry·2026
Same author

Activity-Based Multimodal Probes Uncover the AChE-ADGRB Axis in Cellular and Murine Models of Depression.

Analytical chemistry·2026
Same author

Protein Labeling and Intramolecular Cross-Arylation via Rational Tuning of Bis-Halogenated Pyridiniums.

Analytical chemistry·2026
Same author

Targeted RNA Degradation via LipoSM-RiboTAC Nanoparticles: A Versatile Platform for Cancer Therapy.

Journal of the American Chemical Society·2025
Same author

A Multi-Split-and-Mix Platform for a Variety of Targeted Protein Degradation.

ACS chemical biology·2025
Same author

Sulfur(IV) Chemistry-Based Peptide and Protein Late-Stage Modification.

Chembiochem : a European journal of chemical biology·2025

相关实验视频

Updated: Jun 8, 2025

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

13.9K

可调节的激活 Ester 能够进行氨酸选择性蛋白质标记和分析.

Chuan Wan1, Dongyan Yang2, Yuhao An3

  • 1College of Health Science and Environmental Engineering, Shenzhen Technology University, 518118 Shenzhen, China.

Analytical chemistry
|November 7, 2024
PubMed
概括

研究人员开发了新的化学探针,精确标记蛋白质上的氨酸残留物,克服了它们多种反应性带来的挑战. 这使得我们能够更好地研究蛋白质功能和不可用药物的点.

更多相关视频

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
11:08

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

7.0K
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.2K

相关实验视频

Last Updated: Jun 8, 2025

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

13.9K
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
11:08

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

7.0K
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.2K

科学领域:

  • 化学生物学 化学生物学
  • 蛋白质组学是指蛋白质组学.
  • 有机合成 有机合成

背景情况:

  • 氨酸残留物对蛋白质功能和药物向至关重要,但由于各种微环境,它们表现出不同的反应性.
  • lysine 的特定位点共价变异具有挑战性,阻碍了许多蛋白质的研究,包括不可药物标.

研究的目的:

  • 设计和开发用于蛋白质组内选择性氨酸标记的新型化学探针.
  • 为了克服与氨酸残留的可变pKa值相关的挑战,用于向的共价结合.

主要方法:

  • 通过改变异构原子类型,位置和数量来调节异构芳香激活中的胺反应活性.
  • 系统优化的反应性和选择性用于探头开发.
  • 开发的探针用于体外和细胞溶酶标记和蛋白质组概况的应用.

主要成果:

  • 成功合理化了在异芳香激活中胺基反应活性的调节.
  • 设计和合成了用于选择性lysine标记的有效探针.
  • 证明了这些探针在体外和细胞环境中对蛋白质分析的实用性.

结论:

  • 开发的异芳香激活是一种强大的工具,用于选择性氨酸标签.
  • 这种方法提高了通过精确的标记和分析来研究蛋白质功能和机制的能力.
  • 这些发现为研究无药性蛋白质和推进化学生物学研究提供了新的途径.