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来自基质的环境微生物群可能会干扰基于微生物组的法医相关体液的识别:试点研究

Jun Zhang¹, Daijing Yu¹, Liwei Zhang¹

¹School of Forensic Medicine, Shanxi Medical University, Jinzhong, Shanxi 030600, China; Shanxi Key Laboratory of Forensic Medicine, Jinzhong, Shanxi 030600, China.

Forensic science international. Genetics

|November 7, 2024

概括

犯罪现场基板上的环境微生物显著改变了体液微生物组概况. 消毒方法可以改善法医科学中的基于微生物组的体液识别.

科学领域:

法医微生物学法医微生物学
微生物组的分析
分子生物学分子生物学

背景情况:

微生物组提供了在犯罪现场识别体液的潜力.
基质微生物可以影响基于微生物组的体液识别,但这种影响尚不清楚.

研究的目的:

研究不同基质对沉积体液微生物群的影响.
评估消毒方法在改善从犯罪现场样本中识别体液方面的有效性.

主要方法:

五种体液 (周围血液,月经血液,鼻分泌物,唾液,精液) 沉积在无菌的抹布,床罩和地板上.
微生物群落使用16S rRNA基因安普利康序列分析.
用FEAST分析和PERMANOVA来评估微生物群体的组成和基质/体液的影响.
使用MicroDecon进行了基质微生物消毒.

主要成果:

微生物群落按体液类型聚集在无菌抹布上,但被床罩和地板基板显著改变.
基质微生物影响了模拟体液样本,根据体液类型的影响有所不同.
最初,PERMANOVA表示的基质解释了比体液类型更多的变异 (R2 = 0.211对比0.152).
在去污染后,体液类型解释了比基质 (R2 = 0.108) 更大的差异 (R2 = 0.240).

结论:

来自基板的环境微生物群干扰了基于微生物组的法医相关体液的识别.
消毒策略可以减轻基质效应,增强区分体液类型的能力.
这项研究为在法医调查中应用基于微生物组的染色分析提供了宝贵的见解.

关键词:

清除污染的方法身体液体的识别方法微生物组是一个微生物组.基板基板是一种基板.

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