在Tetrahymena thermophila中,Beta-cypermethrin诱导的应激反应和ABC载体介导的排毒
Wenyong Zhang1, Wenliang Lei2, Tao Bo3
1School of Life Science, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; Taiyuan Institute of Technology, Taiyuan 030008, China.
概括
合成杀虫剂β-cypermethrin (β-CYP) 伤害了水生生物. 热虫表现出压力,但利用排毒途径,ABCG14对β-CYP耐受性至关重要.
科学领域:
- 环境毒理学环境毒理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- β-Cypermethrin (β-CYP) 是一种广泛使用的合成类杀虫剂,已知对水生生态系统产生影响.
- 非目标生物,如单细胞状原生虫Tetrahymena thermophila对环境污染物很敏感.
- 暴露于β-CYP可以改变T.热的增殖和细胞形态.
研究的目的:
- 为了研究β-CYP对T. thermophila的影响.
- 阐明涉及T. thermophila对β-CYP压力的反应中的分子机制和解毒途径.
- 识别关键基因和蛋白质,这些基因和蛋白质有助于T. thermophila的β-CYP耐受性.
主要方法:
- 对T. thermophila暴露于不同度的β-CYP.
- 分析细胞增殖,形态,细胞内活性氧物种 (ROS) 水平和超氧化物脱酶 (SOD) 活性.
- 基因表达概况 (RNA测序) 以确定差异表达的基因.
- 对特定基因进行基因淘汰和过度表达研究,包括ABCG14.
主要成果:
- β-CYP抑制了T.热的增殖和改变了细胞形态.
- 观察到细胞内ROS水平升高和SOD活性升高,而β-CYP度上升.
- 在25mg/Lβ-CYP下,687个基因被上调,富含有机循环和异环化合物结合通路.
- 发现的关键排毒基因包括ATP结合盒 (ABC) 载体,细胞染色体P450单氧化酶和谷氨过氧化酶.
- 在β-CYP压力下,ABCG14的敲击破坏了增殖,而其过度表达增强了耐受性.
结论:
- 热虫采用多种解毒机制,可以耐受高度的β-CYP.
- 结合ATP的磁带载体ABCG14在T. thermophila中对β-CYP的解毒中起着至关重要的作用.
- 了解这些机制可以了解农药对水生微生物的环境影响.
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