碳饥饿驱动了Ganoderma lucidum中高曼诺组成的多糖的生物合成
Mengye Shen1, Mengmeng Xu2, Jingyun Liu1
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China.
International journal of biological macromolecules
|November 7, 2024
概括
光的外聚糖化物 (EPS) 生产可以优化以达到高曼诺含量,这对于抗瘤活性至关重要. 碳饥饿和葡萄糖养策略增强了富含曼的多糖生物合成.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 含有高曼诺斯的多糖具有抗瘤活性.
- 有效制造富含曼的多糖仍然是一个挑战.
- 光 (Ganoderma lucidum) 是一种真菌物种,以产生生物活性多糖而闻名.
研究的目的:
- 为了研究碳利用对外聚糖 (EPS) 生产和单糖组合的碳利用的影响,在Ganoderma lucidum.
- 开发提高高曼诺含量多糖的生物合成的策略.
主要方法:
- 在不同的碳条件下种植Ganoderma lucidum,包括碳饥饿和葡萄糖养.
- 对外聚糖 (EPS) 产量和单糖组成的分析.
- 在GDP-曼诺斯合成途径中的关键酶的基因表达分析.
主要成果:
- 碳饥饿显著增加了EPS中曼的比例,达到35.52%.
- 碳饥饿和葡萄糖养的联合策略产生了0.42g/L的EPS与18.67%的曼诺斯.
- 碳饥饿使GDP-曼诺合成途径中的关键基因 (PMI,PMM,GMP) 的调节提高了两倍以上.
结论:
- 碳利用策略,特别是碳饥饿,可以有效调节Ganoderma lucidum中的EPS单糖化合物组成.
- 开发的联合控制策略为生产高曼诺含量的多糖提供了一种实际方法.
- 这项研究提供了对多糖体生物合成的见解,并支持用于各种应用的Ganoderma lucidum衍生多糖体的开发.
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