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微镜驱动的MALDI成像质谱数据收集工作流的简化工作流程

Allison B Esselman^1,2, Megan S Ward^1,3, Cody R Marshall^1,3

¹Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37240, United States.

Journal of the American Society for Mass Spectrometry

|November 7, 2024

概括

这项研究介绍了在矩阵辅助激光吸附/电离成像质谱学 (MALDI IMS) 中精确组织采样的工作流程. 它通过针对特定感兴趣的区域 (ROI) 来优化数据采集,提高生物医学研究的效率和吞吐量.

科学领域:

生物医学研究的研究.
分子成像学分子成像学
质谱测量质量谱测量

背景情况:

在生物医学研究中,矩阵辅助激光脱/电离成像质谱 (MALDI IMS) 非常重要.
在MALDI IMS中增加空间分辨率会导致更长的获取时间,更大的文件大小和更高的成本.
精确抽样目标组织区域对于优化信息获取和尽量减少资源支出至关重要.

研究的目的:

开发和演示一个工作流程,用于精确的,显微镜引导区域选择在MALDI IMS.
为了实现基于组织特征的特定感兴趣区域 (ROI) 的自动抽样.
为了提高分子成像研究的效率和吞吐量.

主要方法:

开发了一个工作流程,集成标准软件,通过MALDI IMS直接采样显微镜定义的区域.
案例研究包括人脑组织中血管系统的手动注释.
针对脏组织单元和分散的HeLa细胞,使用了自动细分算法.

主要成果:

工作流成功地减少了从不必要的样本区域获取数据.
在实验吞吐量方面取得了显著的增长.
在目标ROI中有效地发现了分子异质性.

结论:

这种方法为面向空间的MALDI IMS提供了一种可访问的方法.
它通过整合显微镜和MALDI IMS来促进多式分子成像研究.
该工作流优化了资源利用,并增强了来自MALDI IMS实验的生物信息检索.

关键词:

这种细胞是HeLa细胞.高空间分辨率成像成像技术高通量的高通量人类大脑人类大脑人类脏人类脏分子成像分子成像技术多式联络方式多式联络一个单细胞分析.有针对性的,有针对性的.有针对性的抽样.

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