使用金属传感器进行定量相位成像内镜
Aamod Shanker1,2, Johannes E Fröch3, Saswata Mukherjee3
1Department of Electrical and Computer Engineering, University of Washington, Seattle, WA, 98195, USA. aamod@berkeley.edu.
Light, science & applications
|November 7, 2024
概括
现在可以在内镜纤维捆的尖端进行定量相成像 (QPI). 这种基于金属的新方法简化了微观成像应用的相恢复.
科学领域:
- 光学成像技术的使用.
- 显微镜的使用方法
- 生物光子学 生物光子学
背景情况:
- 定量相成像 (QPI) 从强度数据重建光波面.
- QPI允许对半透明样品进行地形和光学密度映射.
- 传统的QPI方法在内镜应用中面临挑战,包括复杂的对齐和机械扫描.
研究的目的:
- 为了证明在连贯纤维束的尖端进行定量相位成像.
- 克服当前内镜相位成像技术的局限性.
- 开发一个紧和简化的QPI系统用于内镜使用.
主要方法:
- 采用化超波形金属体,具有固有的色差异.
- 采用光谱多重复合来从多个失焦平面中恢复相位信息,在单个摄像头捕捉中.
- 集成金属作为一个聚焦元件和光谱过器为0.5毫米孔径系统.
主要成果:
- 实现了强大的定量相位成像,视野和0. 阶段分辨率 (大约. 0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0.0. 在空气中).
- 通过内镜纤维捆绑证明了QPI的能力.
- 基于金属的系统减轻了对多次采集,干扰度对齐或机械扫描的需求.
结论:
- 基于金属的定量相位成像系统可用于内镜应用.
- 开发的方法通过利用固有的染色异常和光谱复杂化来简化QPI.
- 该系统的设计能够通过简单的计算后端实现实时操作,为先进的内镜成像铺平了道路.
更多相关视频
09:42Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
Published on: August 26, 2014
18.6K
14:09Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
15.5K
相关概念视频
Phase Contrast and Differential Interference Contrast Microscopy
7.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.5K
Endoscopic Procedures III: Video Capsule Endoscopy
81
Capsule endoscopy, or wireless or video capsule endoscopy, is a diagnostic procedure for examining the entire gastrointestinal tract. Patients swallow a capsule about the size of a vitamin tablet. The capsule is equipped with a transmitter, a battery, an LED light source, and a color video camera to capture images throughout the gastrointestinal tract. This procedure is particularly useful for diagnosing conditions such as Crohn's disease, ulcerative colitis, tumors, polyps, ulcers,...
81
