相关实验视频
Updated: Jun 8, 2025
09:20
CRISPR-Mediated Reorganization of Chromatin Loop Structure
Published on: September 14, 2018
12.5K
BAF复合体通过与H3K56ac在基因素球状域中的相互作用来增强转录
Kwangbeom Hyun1, Jihye Ahn1, Hyoungmin Kim1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea.
Nature communications
|November 7, 2024
概括
染色体效应蛋白通过与基因组球状域修饰相互作用来调节基因表达. 该BAF复合体与H3K56ac结合,增强转录和DNA损伤反应.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因规则 基因规则
背景情况:
- 基因组转换后的修改对于真核生物的基因表达至关重要.
- 研究主要集中在N-终端尾部修饰,忽视球状域角色.
- 通过基因组球状域修改调节转录的染色质因子直接证据有限.
研究的目的:
- 为了研究基因组球状域修饰在转录调节中的作用.
- 为了确定BAF复合体是否与基因组球状域修饰相互作用.
- 阐明BAF复合体影响基因表达的机制.
主要方法:
- 生物化学试验检测BAF复合物和修饰的组织蛋白之间的相互作用.
- 核细胞重塑试验用于评估BAF复杂活性.
- 分析p53依赖转录和DNA损伤反应在细胞与改变的H3K56ac-BAF相互作用.
主要成果:
- 依赖ATP的染色体重塑BAF复合体直接与基因组球状域上的H3K56ac相互作用.
- DPF2子单元的DPF域介导BAF复杂识别H3K56ac.的复杂识别.
- 结合H3K56ac增强了BAF复杂核细胞重塑活动.
- 损坏的H3K56ac-BAF相互作用会破坏p53依赖的基因表达和DNA损伤反应.
结论:
- 基因组球状域的修改,特别是H3K56ac,直接调节基因表达.
- BAF复合体利用H3K56ac进行转录刺激和DNA损伤反应.
- 这项研究建立了一个新的基因调节机制,涉及基因组球状域修饰.
相关概念视频
02:32Histone Modification
13.1K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.1K
02:25Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K
01:12The Nucleosome Core Particle
880
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
880
02:38Heterochromatin
11.4K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
11.4K
01:19The Nucleosome
1.3K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
1.3K
02:30Histone Variants at the Centromere
4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K