乙型肝炎病毒RNA在血和血清中的稳定性有限
Valerie Ohlendorf1, Birgit Bremer1, Lisa Sandmann1
1Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, Hannover, Germany.
Scientific reports
|November 7, 2024
概括
乙型肝炎病毒RNA (HBV pgRNA) 的稳定性对于其作为生物标记物的使用至关重要. 对于稳定的定量HBVpgRNA,建议在4°C储存,而在4-42°C时,定性检测可能长达48小时.
科学领域:
- 病毒学 病毒学
- 分子诊断学 分子诊断
- 临床化学 临床化学
背景情况:
- 前基因乙型肝炎病毒RNA (HBV pgRNA) 显示出作为管理HBV感染的生物标志物具有前景.
- 在例行临床应用之前,识别潜在的混因素,例如储存条件,至关重要.
研究的目的:
- 为了研究HBVpgRNA在各种储存温度和冷解周期下的稳定性.
- 为了确定最佳的储存条件,以保持HBV pgRNA完整性,用于诊断目的.
主要方法:
- 在26名患者的HBV pgRNA水平使用罗氏cobas® 6800/8800试验量化.
- 血和血清样本在4°C,25°C和42°C下存储长达169小时.
- 额外的样本经历了多次冷解周期 (-80°C至25°C).
主要成果:
- 在25°C下48小时,在42°C下6小时后,HBV pgRNA度显著下降.
- 储存温度显著影响pgRNA水平,特别是在基线度较低的样本中.
- 在重复冷和解周期后,没有发现对pgRNA水平的显著影响.
结论:
- 对于最稳定的定量HBVpgRNA测量,建议在4°C储存.
- 在4°C至42°C的温度下保存长达48小时的>100 cp/mL的样本中,可以对HBV pgRNA进行定性检测.
- 了解HBV pgRNA稳定性对于可靠的临床治疗乙型肝炎病毒感染至关重要.
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