在2岁的海上松树中,体质胚胎成熟期间感知到的温度的表观遗传记忆
Jean-François Trontin1, Mamadou Dia Sow2, Alain Delaunay2
1BioForBois, FCBA, Pôle Industrie Bois & Construction, Cestas 33610, France.
Plant physiology
|November 8, 2024
概括
树胚胎发育过程中的温度会产生持久的表观遗传记忆. 这种DNA甲基化变化影响胚胎后生长,影响海上松树的适应和防御反应.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 林业林业 林业 林业 林业
背景情况:
- 胚胎发生是树木适应性可塑性的关键阶段.
- 海洋松体胚胎发生过程中的温度压力会影响后来的树木生长.
- 表观遗传修饰,特别是DNA甲基化,被研究为这种压力记忆的机制.
研究的目的:
- 调查胚胎发生过程中温度和发育变化是否会诱导DNA甲基化变化.
- 确定这些DNA甲基化变化是否从胚胎到胚胎后阶段通过线粒传播 (表观遗传记忆).
- 确定参与海洋松树温度持续表观遗传记忆的候选基因.
主要方法:
- 在海上松树 (Pinus pinaster Ait. ) 的情况.
- 定制序列捕获对基因和促进体的双硫酸盐分析.
- 在温度处理和发育阶段对差异甲基化细胞因子 (DMCs) 的分析.
主要成果:
- 基因中温度诱导的DMCs的高患病率 (8-14%) 在所有细胞因子环境中.
- 发育期的DMC在促进剂中表现出低甲基化趋势 (CG背景).
- 显著的发育性DMC的传播 (在促进者中高达90%) 和较低温度诱导的记忆 (在基因中高达14%).
结论:
- 在胚胎发育过程中建立的DNA甲基化标记被传递到胚胎后阶段.
- 确定了10个参与防御,适应和染色质调节的候选基因,可能调解表观遗传记忆.
- 在海松胚胎成熟过程中经历的温度可以建立持续的表观遗传记忆,影响树木发育.
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